Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2021 Jun 2

PMID 34073956

Citations 2

Authors

Cory Juntunen

Isabel M Woller

Yongjin Sung

Affiliations

Soon will be listed here.

Abstract

Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.

Citing Articles

Hyperspectral three-dimensional absorption imaging using snapshot optical tomography.

Juntunen C, Abramczyk A, Woller I, Sung Y Phys Rev Appl. 2023; 18(3).

PMID: 37274485 PMC: 10237288. DOI: 10.1103/physrevapplied.18.034055.

Optical projection tomography of fluorescent microscopic specimens using lateral translation of tube lens.

Sung Y Opt Lett. 2023; 48(10):2623-2626.

PMID: 37186724 PMC: 10798857. DOI: 10.1364/OL.491499.

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