Ancient Bacterial Class Cytochrome P450 Monooxygenases Can Be Found in Other Bacterial Species

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Jun 2

PMID 34073951

Citations 6

Authors

Nomfundo Nzuza

Tiara Padayachee

Puleng Rosinah Syed

Justyna Dorota Krys

Wanping Chen

Dominik Gront

David R Nelson

Khajamohiddin Syed

Affiliations

Soon will be listed here.

Abstract

Cytochrome P450 monooxygenases (CYPs/P450s), heme-thiolate proteins, are well-known players in the generation of chemicals valuable to humans and as a drug target against pathogens. Understanding the evolution of P450s in a bacterial population is gaining momentum. In this study, we report comprehensive analysis of P450s in the ancient group of the bacterial class . Genome data mining and annotation of P450s in 599 alphaproteobacterial species belonging to 164 genera revealed the presence of P450s in only 241 species belonging to 82 genera that are grouped into 143 P450 families and 214 P450 subfamilies, including 77 new P450 families. Alphaproteobacterial species have the highest average number of P450s compared to species and cyanobacterial species. The lowest percentage of alphaproteobacterial species P450s (2.4%) was found to be part of secondary metabolite biosynthetic gene clusters (BGCs), compared other bacterial species, indicating that during evolution large numbers of P450s became part of BGCs in other bacterial species. Our study identified that some of the P450 families found in alphaproteobacterial species were passed to other bacterial species. This is the first study to report on the identification of CYP125 P450, cholesterol and cholest-4-en-3-one hydroxylase in alphaproteobacterial species () and to predict cholesterol side-chain oxidation capability (based on homolog proteins) by .

Citing Articles

Cytochromes P450 involved in bacterial RiPP biosyntheses.

Kunakom S, Otani H, Udwary D, Doering D, Mouncey N J Ind Microbiol Biotechnol. 2023; 50(1).

PMID: 36931895 PMC: 10124130. DOI: 10.1093/jimb/kuad005.

Saprophytic to Pathogenic Mycobacteria: Loss of Cytochrome P450s Vis a Vis Their Prominent Involvement in Natural Metabolite Biosynthesis.

Zondo N, Padayachee T, Nelson D, Syed K Int J Mol Sci. 2023; 24(1).

PMID: 36613600 PMC: 9820752. DOI: 10.3390/ijms24010149.

An Unprecedented Number of Cytochrome P450s Are Involved in Secondary Metabolism in Species.

Malinga N, Nzuza N, Padayachee T, Syed P, Karpoormath R, Gront D Microorganisms. 2022; 10(5).

PMID: 35630316 PMC: 9143469. DOI: 10.3390/microorganisms10050871.

Lifestyles Shape the Cytochrome P450 Repertoire of the Bacterial Phylum .

Msweli S, Chonco A, Msweli L, Syed P, Karpoormath R, Chen W Int J Mol Sci. 2022; 23(10).

PMID: 35628630 PMC: 9148083. DOI: 10.3390/ijms23105821.

Contrasting Health Effects of and Lies in Their Genomes: Analysis of P450s, Ferredoxins, and Secondary Metabolite Clusters.

Nkosi B, Padayachee T, Gront D, Nelson D, Syed K Int J Mol Sci. 2022; 23(9).

PMID: 35563448 PMC: 9100364. DOI: 10.3390/ijms23095057.

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