A Non-Invasive Hair Test to Determine Vitamin D Levels

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Jun 2

PMID 34071612

Citations 3

Authors

Iltaf Shah

Mohammad Mansour

Sheikh Jobe

Emadaldeen Salih

Declan Naughton

Syed Salman Ashraf

Affiliations

Soon will be listed here.

Abstract

Vitamin D deficiency is being recognized as a global issue and has been implicated in many health issues. Hence, there is an increased interest in developing sensitive, reproducible, and non-invasive assays to measure Vitamin D levels. This study aimed to apply a sensitive liquid chromatography-mass spectrometric assay to hair samples to develop and validate a clinical assay to provide a quarterly average level of vitamin D in one test. Hair samples were collected from 70 male university students/young adults and pulverized/sonicated in methanol/water for 2 h to extract Vitamin D metabolites. A sensitive liquid chromatographic-mass spectrometric assay was employed to quantitate vitamin D and metabolites. Of the eight Vitamin D and metabolites screened, only the primary, clinically significant form of vitamin D (25OHD) was detected and quantified in hair samples in the range of 17-1541 pg/mg. One-third of the hair samples (21 out of 70) had Vitamin D levels below the LLOD of the assay (10 pg/mg). The mean and standard deviation values for hair (25OHD) were 276.7 ± 329.9, respectively. This pilot study reveals the potential of the vitamin D hair test in clinical assays as a complementary test to a vitamin D blood test, which would provide a quarterly average.

Citing Articles

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References

Sattar N, Welsh P, Panarelli M, Forouhi N . Increasing requests for vitamin D measurement: costly, confusing, and without credibility. Lancet. 2012; 379(9811):95-6. DOI: 10.1016/S0140-6736(11)61816-3. View

Shah I, Al-Dabbagh B, Gariballa S, Al-Menhali A, Muhammad N, Yasin J . Application of a new vitamin D blood test on the Emirati population. J Steroid Biochem Mol Biol. 2018; 180:118-128. DOI: 10.1016/j.jsbmb.2018.02.003. View

Ross A, Manson J, Abrams S, Aloia J, Brannon P, Clinton S . The 2011 report on dietary reference intakes for calcium and vitamin D from the Institute of Medicine: what clinicians need to know. J Clin Endocrinol Metab. 2010; 96(1):53-8. PMC: 3046611. DOI: 10.1210/jc.2010-2704. View

Zimmer D . New US FDA draft guidance on bioanalytical method validation versus current FDA and EMA guidelines: chromatographic methods and ISR. Bioanalysis. 2013; 6(1):13-9. DOI: 10.4155/bio.13.298. View

Cashman K, Kinsella M, Walton J, Flynn A, Hayes A, Lucey A . The 3 epimer of 25-hydroxycholecalciferol is present in the circulation of the majority of adults in a nationally representative sample and has endogenous origins. J Nutr. 2014; 144(7):1050-7. PMC: 4056645. DOI: 10.3945/jn.114.192419. View

Zhang R, Naughton D . Vitamin D in health and disease: current perspectives. Nutr J. 2010; 9:65. PMC: 3019131. DOI: 10.1186/1475-2891-9-65. View

Lensmeyer G, Poquette M, Wiebe D, Binkley N . The C-3 epimer of 25-hydroxyvitamin D(3) is present in adult serum. J Clin Endocrinol Metab. 2011; 97(1):163-8. DOI: 10.1210/jc.2011-0584. View

Jenkinson C, Taylor A, Hassan-Smith Z, Adams J, Stewart P, Hewison M . High throughput LC-MS/MS method for the simultaneous analysis of multiple vitamin D analytes in serum. J Chromatogr B Analyt Technol Biomed Life Sci. 2016; 1014:56-63. PMC: 4801106. DOI: 10.1016/j.jchromb.2016.01.049. View

Sirajudeen S, Shah I, Menhali A . A Narrative Role of Vitamin D and Its Receptor: With Current Evidence on the Gastric Tissues. Int J Mol Sci. 2019; 20(15). PMC: 6695859. DOI: 10.3390/ijms20153832. View

10.

Zgaga L, Laird E, Healy M . 25-Hydroxyvitamin D Measurement in Human Hair: Results from a Proof-of-Concept study. Nutrients. 2019; 11(2). PMC: 6412768. DOI: 10.3390/nu11020423. View

11.

Yetley E . Assessing the vitamin D status of the US population. Am J Clin Nutr. 2008; 88(2):558S-564S. DOI: 10.1093/ajcn/88.2.558S. View

12.

Al-Zohily B, Al-Menhali A, Gariballa S, Haq A, Shah I . Epimers of Vitamin D: A Review. Int J Mol Sci. 2020; 21(2). PMC: 7013384. DOI: 10.3390/ijms21020470. View

13.

Karras S, Shah I, Petroczi A, Goulis D, Bili H, Papadopoulou F . An observational study reveals that neonatal vitamin D is primarily determined by maternal contributions: implications of a new assay on the roles of vitamin D forms. Nutr J. 2013; 12:77. PMC: 3680300. DOI: 10.1186/1475-2891-12-77. View

14.

Higashi T, Awada D, Shimada K . Simultaneous determination of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma by liquid chromatography-tandem mass spectrometry employing derivatization with a Cookson-type reagent. Biol Pharm Bull. 2001; 24(7):738-43. DOI: 10.1248/bpb.24.738. View

15.

Shah I, Petroczi A, Naughton D . Method for simultaneous analysis of eight analogues of vitamin D using liquid chromatography tandem mass spectrometry. Chem Cent J. 2012; 6(1):112. PMC: 3505479. DOI: 10.1186/1752-153X-6-112. View

16.

Heaney R . Assessing vitamin D status. Curr Opin Clin Nutr Metab Care. 2011; 14(5):440-4. DOI: 10.1097/MCO.0b013e328348ed85. View

17.

Molnar F, Sigueiro R, Sato Y, Araujo C, Schuster I, Antony P . 1α,25(OH)2-3-epi-vitamin D3, a natural physiological metabolite of vitamin D3: its synthesis, biological activity and crystal structure with its receptor. PLoS One. 2011; 6(3):e18124. PMC: 3069065. DOI: 10.1371/journal.pone.0018124. View

18.

Shah I, Petroczi A, Naughton D . Exploring the role of vitamin D in type 1 diabetes, rheumatoid arthritis, and Alzheimer disease: new insights from accurate analysis of 10 forms. J Clin Endocrinol Metab. 2014; 99(3):808-16. DOI: 10.1210/jc.2013-2872. View

19.

Spiro A, Buttriss J . Vitamin D: An overview of vitamin D status and intake in Europe. Nutr Bull. 2015; 39(4):322-350. PMC: 4288313. DOI: 10.1111/nbu.12108. View

20.

Holick M . Vitamin D status: measurement, interpretation, and clinical application. Ann Epidemiol. 2008; 19(2):73-8. PMC: 2665033. DOI: 10.1016/j.annepidem.2007.12.001. View