Optogenetic Approaches for the Spatiotemporal Control of Signal Transduction Pathways

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Jun 2

PMID 34069904

Citations 11

Authors

Markus M Kramer

Levin Lataster

Wilfried Weber

Gerald Radziwill

Affiliations

Soon will be listed here.

Abstract

Biological signals are sensed by their respective receptors and are transduced and processed by a sophisticated intracellular signaling network leading to a signal-specific cellular response. Thereby, the response to the signal depends on the strength, the frequency, and the duration of the stimulus as well as on the subcellular signal progression. Optogenetic tools are based on genetically encoded light-sensing proteins facilitating the precise spatiotemporal control of signal transduction pathways and cell fate decisions in the absence of natural ligands. In this review, we provide an overview of optogenetic approaches connecting light-regulated protein-protein interaction or caging/uncaging events with steering the function of signaling proteins. We briefly discuss the most common optogenetic switches and their mode of action. The main part deals with the engineering and application of optogenetic tools for the control of transmembrane receptors including receptor tyrosine kinases, the T cell receptor and integrins, and their effector proteins. We also address the hallmarks of optogenetics, the spatial and temporal control of signaling events.

Citing Articles

Optogenetic Control of the Mitochondrial Protein Import in Mammalian Cells.

Althoff L, Kramer M, Buhrer B, Gaspar D, Radziwill G Cells. 2024; 13(19.

PMID: 39404433 PMC: 11482626. DOI: 10.3390/cells13191671.

Optogenetic therapeutic strategies for diabetes mellitus.

Deng X, Peng D, Yao Y, Huang K, Wang J, Ma Z J Diabetes. 2024; 16(6):e13557.

PMID: 38751366 PMC: 11096815. DOI: 10.1111/1753-0407.13557.

A single-component, light-assisted uncaging switch for endoproteolytic release.

Cui M, Lee S, Ban S, Ryu J, Shen M, Yang S Nat Chem Biol. 2023; 20(3):353-364.

PMID: 37973890 DOI: 10.1038/s41589-023-01480-6.

Engineering global and local signal generators for probing temporal and spatial cellular signaling dynamics.

Yang H, Tel J Front Bioeng Biotechnol. 2023; 11:1239026.

PMID: 37790255 PMC: 10543096. DOI: 10.3389/fbioe.2023.1239026.

Cell Cycle Control by Optogenetically Regulated Cell Cycle Inhibitor Protein p21.

Lataster L, Huber H, Bottcher C, Foller S, Takors R, Radziwill G Biology (Basel). 2023; 12(9).

PMID: 37759593 PMC: 10525493. DOI: 10.3390/biology12091194.

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