MiR155 Deficiency Reduces Myofibroblast Density but Fails to Improve Cardiac Function After Myocardial Infarction in Dyslipidemic Mouse Model
Overview
Chemistry
Molecular Biology
Authors
Affiliations
Myocardial infarction remains the most common cause of heart failure with adverse remodeling. MicroRNA (miR)155 is upregulated following myocardial infarction and represents a relevant regulatory factor for cardiac remodeling by engagement in cardiac inflammation, fibrosis and cardiomyocyte hypertrophy. Here, we investigated the role of miR155 in cardiac remodeling and dysfunction following myocardial infarction in a dyslipidemic mouse model. Myocardial infarction was induced in dyslipidemic apolipoprotein E-deficient (ApoE) mice with and without additional miR155 knockout by ligation of the LAD. Four weeks later, echocardiography was performed to assess left ventricular (LV) dimensions and function, and mice were subsequently sacrificed for histological analysis. Echocardiography revealed no difference in LV ejection fractions, LV mass and LV volumes between ApoE and ApoE/miR155 mice. Histology confirmed comparable infarction size and unaltered neoangiogenesis in the myocardial scar. Notably, myofibroblast density was significantly decreased in ApoE/miR155 mice compared to the control, but no difference was observed for total collagen deposition. Our findings reveal that genetic depletion of miR155 in a dyslipidemic mouse model of myocardial infarction does not reduce infarction size and consecutive heart failure but does decrease myofibroblast density in the post-ischemic scar.
Noncoding RNAs and Cardiac Fibrosis.
Wu C, Bao S, Li R, Sun H, Peng Y Rev Cardiovasc Med. 2024; 24(2):63.
PMID: 39077397 PMC: 11273127. DOI: 10.31083/j.rcm2402063.
The Role of ncRNAs in Cardiac Infarction and Regeneration.
Cano-Carrillo S, Lozano-Velasco E, Castillo-Casas J, Sanchez-Fernandez C, Franco D J Cardiovasc Dev Dis. 2023; 10(3).
PMID: 36975887 PMC: 10052289. DOI: 10.3390/jcdd10030123.
Novel Biomarkers of Atherosclerotic Vascular Disease-Latest Insights in the Research Field.
Adam C, Salaru D, Prisacariu C, Marcu D, Sascau R, Statescu C Int J Mol Sci. 2022; 23(9).
PMID: 35563387 PMC: 9103799. DOI: 10.3390/ijms23094998.
MiR-410-3p facilitates Angiotensin II-induced cardiac hypertrophy by targeting Smad7.
Jia G, Liang C, Li W, Dai H Bioengineered. 2021; 13(1):119-127.
PMID: 34951337 PMC: 8805929. DOI: 10.1080/21655979.2021.2009968.
Heart function assessment during aging in apolipoprotein E knock-out mice.
Liehn E, Lupan A, Diaconu R, Ioana M, Streata I, Manole C Discoveries (Craiova). 2021; 9(3):e136.
PMID: 34816004 PMC: 8605688. DOI: 10.15190/d.2021.15.