OBP14 (Odorant-Binding Protein) Sensing in Based on Peptide Nucleic Acid and Graphene Oxide

Overview

Journal Insects

Specialty Biology

Date 2021 Jun 2

PMID 34066819

Citations 3

Authors

Wenhua Tian

Tao Zhang

Shaohua Gu

Yuyuan Guo

Xiwu Gao

Yongjun Zhang

Affiliations

Soon will be listed here.

Abstract

OBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in the physiological functions of Plus-C OBPs remain largely unknown. In addition, detection of the expression of insect OBP genes by conventional methods is difficult in vitro. Here, we focused on , a Plus-C OBP from and we developed a PNA-GO-based mRNA biosensor to detect the expression of . The results demonstrated that plays dual roles in . The is expressed beneath the epidermis of the vertex and gena in heads of and it functions as a carrier for three terpenoids, while is also expressed in the peripheral antennal lobe and functions as a carrier for endogenous compounds such as precursors for juvenile hormone (JH) and JHⅢ. Our investigation provides a new method to detect the expression of OBP genes in insects, and the technique will facilitate the use of these genes as potential targets for novel insect behavioral regulation strategies against the pest.

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