Isolation of Cell-Free MiRNA from Biological Fluids: Influencing Factors and Methods

Overview

Journal Diagnostics (Basel)

Specialty Radiology

Date 2021 Jun 2

PMID 34064927

Citations 19

Authors

Olga Bryzgunova

Maria Konoshenko

Ivan Zaporozhchenko

Alexey Yakovlev

Pavel Laktionov

Affiliations

Soon will be listed here.

Abstract

A vast wealth of recent research has seen attempts of using microRNA (miRNA) found in biological fluids in clinical research and medicine. One of the reasons behind this trend is the apparent their high stability of cell-free miRNA conferred by small size and packaging in supramolecular complexes. However, researchers in both basic and clinical settings often face the problem of selecting adequate methods to extract appropriate quality miRNA preparations for use in specific downstream analysis pipelines. This review outlines the variety of different methods of miRNA isolation from biofluids and examines the key determinants of their efficiency, including, but not limited to, the structural properties of miRNA and factors defining their stability in the extracellular environment.

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