Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method

Overview

Journal Cells

Publisher MDPI

Specialties Biochemistry
Biophysics
Cell Biology
Molecular Biology

Date 2021 Jun 2

PMID 34063138

Citations 8

Authors

Lukas Prantl

Andreas Eigenberger

Eva Brix

Sally Kempa

Magnus Baringer

Oliver Felthaus

Affiliations

Soon will be listed here.

Abstract

In plastic surgery, lipofilling is a frequent procedure. Unsatisfactory vascularization and impaired cell vitality can lead to unpredictable take rates in the fat graft. The proliferation and neovascularization inducing properties of adipose tissue-derived stem cells may contribute to solve this problem. Therefore, the enrichment of fat grafts with stem cells is studied intensively. However, it is difficult to compare these studies because many factors-often not precisely described-are influencing the results. Our study summarizes some factors which influence the cell yield like harvesting, isolation procedure and quantification. Stem cells were isolated after liposuction. Quantification was done using a cell chamber, colony counting, or flow cytometry with changes to one parameter, only, for each comparison. Quantification of cells isolated after liposuction at the same harvesting site from the same patient can vary greatly depending on the details of the isolation protocol and the method of quantification. Cell yield can be influenced strongly by many factors. Therefore, a comparison of different studies should be handled with care.

Citing Articles

Breast Lipofilling: Is the Bra Really Full? Clinical Bra Pressure Measurement and In Vitro Testing of Processed and Unprocessed Fat Cells.

Biermann N, Eigenberger A, Felthaus O, Brebant V, Heine N, Brix E Aesthetic Plast Surg. 2024; 48(23):5018-5025.

PMID: 38814346 DOI: 10.1007/s00266-024-04116-9.

Cell-Enriched Lipotransfer (CELT) Improves Tissue Regeneration and Rejuvenation without Substantial Manipulation of the Adipose Tissue Graft.

Prantl L, Eigenberger A, Reinhard R, Siegmund A, Heumann K, Felthaus O Cells. 2022; 11(19).

PMID: 36231121 PMC: 9563290. DOI: 10.3390/cells11193159.

The Effects of Shear Force-Based Processing of Lipoaspirates on White Adipose Tissue and the Differentiation Potential of Adipose Derived Stem Cells.

Eigenberger A, Felthaus O, Schratzenstaller T, Haerteis S, Utpatel K, Prantl L Cells. 2022; 11(16).

PMID: 36010620 PMC: 9406387. DOI: 10.3390/cells11162543.

Mesenchymal Stem Cell Mechanisms of Action and Clinical Effects in Osteoarthritis: A Narrative Review.

Molnar V, Pavelic E, Vrdoljak K, cemerin M, Klaric E, Matisic V Genes (Basel). 2022; 13(6).

PMID: 35741711 PMC: 9222975. DOI: 10.3390/genes13060949.

Distinct Dominant Lineage from In Vitro Expanded Adipose-Derived Stem Cells (ASCs) Exhibits Enhanced Wound Healing Properties.

Peng Q, Ren G, Xuan Z, Duda M, Pennisi C, Porsborg S Cells. 2022; 11(7).

PMID: 35406800 PMC: 8998068. DOI: 10.3390/cells11071236.

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