Comparative Analysis of Routine Parasitological Methods for Recovery of Cysts, Molecular Detection, and Genotyping of Giardia Duodenalis

Overview

Journal Eur J Clin Microbiol Infect Dis

Publisher Springer

Specialties Infectious Diseases
Microbiology

Date 2021 Jun 1

PMID 34059933

Authors

Renata Coltro Bezagio

Cristiane Maria Colli

Liara Izabela Lopes Romera

Caroline Rodrigues de Almeida

Erika Cristina Ferreira

Monica Lucia Gomes

Affiliations

Soon will be listed here.

Abstract

In order to improve the diagnosis of giardiasis, fecal samples (high/medium/low concentration of cysts) were processed by the parasitological methods used in the routine: Faust, Lutz e Ritchie modified (replacement of formaldehyde by distilled water). The cysts were quantified; the DNA was extracted and amplified by semi-nested PCR (GDH gene). Fifteen clinical samples were analyzed to validate the study by PCR-RFLP. The results showed that the parasite was only detected and genotyped correctly when samples from children with high, medium, and low parasitic load, belonging to genotype AII, were processed by the modified Ritchie method, different from what was observed for the other methods used in laboratory routine (Faust and Lutz). The modified Ritchie method proved to be more suitable, recovering a greater number of cysts from samples, regardless of parasitic load, which reduces the chance of false negative results and has epidemiological repercussions since individuals with low parasite load are usually asymptomatic and the main disseminators of this infection.

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