Digital Analysis of Subtrochlear Sclerosis in Elbows Submitted for Dysplasia Screening

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2021 May 31

PMID 34055952

Authors

Ana Valega

Sofia Alves-Pimenta

Fintan J McEvoy

Dorte H Nielsen

Mario Ginja

Affiliations

Soon will be listed here.

Abstract

Ulnar trochlear notch (UTN) subchondral bone sclerosis is observed in elbow dysplasia (ED) associated with the medial coronoid disease. However, its evaluation is based on a simple visual examiner assessment of bone radio-opacity level and is considered subjective. The purpose of this study was to objectively characterize the radiographic opacity of the ulnar trochlear notch (UTN) subchondral bone in mediolateral elbow projections classified, using the International Elbow Working Group guidelines. Records and mediolateral flexed elbow images from the Danish Kennel Club database for the ED screening scheme between 2012 and 2018 were available. Of the dogs in the database, those with an ED-negative status in the left limb were identified. From these, 20 dogs each having a status free from ED, or with Grade 1, 2, or 3 in the right limb, were randomly chosen. Joints with primary ununited anconeal process were excluded from the sample. A template was developed using the ImageJ software, for computer UTN sclerosis analysis. It was overlaid onto each image to define five regions of interest (ROIs): ROI-1, distal UTN; ROI-2, middle UTN; ROI-3, caudal UTN; ROI-4, cortical bone; and ROI-5, bone marrow. Mean pixel intensity for each UTN ROI was divided by the mean pixel intensity of ROI-4 to normalize the data. The mean ± standard deviation (SD) of the normalized pixel intensity in the disease joints (ED Grades 1, 2, and 3) was 1.18 ± 0.17, 1.03 ± 0.12, and 0.92 ± 0.09 for ROIs 1, 2, and 3, respectively. The corresponding values for the contralateral normal left joints were 1.16 ± 0.17, 1.01 ± 0.1, and 0.91 ± 0.08. There was a significant difference ( < 0.05) in the normalized mean pixel intensity in dysplastic vs. non-dysplastic elbow joints for ROIs 1 and 2. The raw mean pixel intensity from right and left cortical and marrow bone ROIs sometimes showed relatively large differences. Digital radiography is associated with exposure and post-processing variabilities. Differences in apparent radio-opacity (as indicated by pixel intensity) though statistically significant in dysplastic joints compared with contralateral normal joints are slight and are thus problematic for computer-aided assessments of UTN sclerosis.

References

Burton N, Perry M, Fitzpatrick N, Owen M . Comparison of bone mineral density in medial coronoid processes of dogs with and without medial coronoid process fragmentation. Am J Vet Res. 2010; 71(1):41-6. DOI: 10.2460/ajvr.71.1.41. View

ONeill D, Brodbelt D, Hodge R, Church D, Meeson R . Epidemiology and clinical management of elbow joint disease in dogs under primary veterinary care in the UK. Canine Med Genet. 2020; 7:1. PMC: 7371807. DOI: 10.1186/s40575-020-0080-5. View

Draffan D, Carrera I, Carmichael S, Heller J, Hammond G . Radiographic analysis of trochlear notch sclerosis in the diagnosis of osteoarthritis secondary to medial coronoid disease. Vet Comp Orthop Traumatol. 2009; 22(1):7-15. View

Phillips A, Burton N, Warren-Smith C, Kulendra E, Parsons K . Topographic bone density of the radius and ulna in greyhounds and labrador retrievers with and without medial coronoid process disease. Vet Surg. 2014; 44(2):180-90. DOI: 10.1111/j.1532-950X.2014.12294.x. View

Lau S, Wolschrijn C, Siebelt M, Vernooij J, Voorhout G, Hazewinkel H . Assessment of articular cartilage and subchondral bone using EPIC-microCT in Labrador retrievers with incipient medial coronoid disease. Vet J. 2013; 198(1):116-21. DOI: 10.1016/j.tvjl.2013.05.038. View

Lavrijsen I, Heuven H, Voorhout G, Meij B, Theyse L, Leegwater P . Phenotypic and genetic evaluation of elbow dysplasia in Dutch Labrador Retrievers, Golden Retrievers, and Bernese Mountain dogs. Vet J. 2012; 193(2):486-92. DOI: 10.1016/j.tvjl.2012.01.001. View

Smith T, Fitzpatrick N, Evans R, Pead M . Measurement of ulnar subtrochlear sclerosis using a percentage scale in labrador retrievers with minimal radiographic signs of periarticular osteophytosis. Vet Surg. 2009; 38(2):199-208. DOI: 10.1111/j.1532-950X.2008.00488.x. View

Burton N, Toscano M, Barr F, Owen M . Reliability of radiological assessment of ulnar trochlear notch sclerosis in dysplastic canine elbows. J Small Anim Pract. 2008; 49(11):572-6. DOI: 10.1111/j.1748-5827.2008.00611.x. View

Lau S, Theyse L, Voorhout G, Hazewinkel H . Radiographic, computed tomographic, and arthroscopic findings in labrador retrievers with medial coronoid disease. Vet Surg. 2014; 44(4):511-20. DOI: 10.1111/j.1532-950X.2014.12291.x. View

10.

James H, McDonnell F, Lewis T . Effectiveness of Canine Hip Dysplasia and Elbow Dysplasia Improvement Programs in Six UK Pedigree Breeds. Front Vet Sci. 2020; 6:490. PMC: 6974481. DOI: 10.3389/fvets.2019.00490. View

11.

Dickomeit M, Bottcher P, Hecht S, Liebich H, Maierl J . Topographic and age-dependent distribution of subchondral bone density in the elbow joints of clinically normal dogs. Am J Vet Res. 2011; 72(4):491-9. DOI: 10.2460/ajvr.72.4.491. View

12.

UBBINK G, Hazewinkel H, van de Broek J, Rothuizen J . Familial clustering and risk analysis for fragmented coronoid process and elbow joint incongruity in Bernese Mountain Dogs in The Netherlands. Am J Vet Res. 1999; 60(9):1082-7. View

13.

Villamonte-Chevalier A, Dingemanse W, Broeckx B, Van Caelenberg A, Agut A, Duchateau L . Bone density of elbow joints in Labrador retrievers and Golden retrievers: Comparison of healthy joints and joints with medial coronoid disease. Vet J. 2016; 216:1-7. DOI: 10.1016/j.tvjl.2016.06.005. View

14.

Moorman L, Precht H, Jensen J, Svalastoga E, Nielsen D, Proschowsky H . Assessment of Image Quality in Digital Radiographs Submitted for Hip Dysplasia Screening. Front Vet Sci. 2019; 6:428. PMC: 6901622. DOI: 10.3389/fvets.2019.00428. View

15.

Baers G, Keller G, Famula T, Oberbauer A . Heritability of Unilateral Elbow Dysplasia in the Dog: A Retrospective Study of Sire and Dam Influence. Front Vet Sci. 2019; 6:422. PMC: 6883289. DOI: 10.3389/fvets.2019.00422. View

16.

Lappalainen A, Molsa S, Liman A, Laitinen-Vapaavuori O, Snellman M . Radiographic and computed tomography findings in Belgian shepherd dogs with mild elbow dysplasia. Vet Radiol Ultrasound. 2009; 50(4):364-9. DOI: 10.1111/j.1740-8261.2009.01551.x. View

17.

Burton N, Comerford E, Bailey M, Pead M, Owen M . Digital analysis of ulnar trochlear notch sclerosis in Labrador retrievers. J Small Anim Pract. 2007; 48(4):220-4. DOI: 10.1111/j.1748-5827.2006.00259.x. View