Separation of Heat-Stable Antifungal Factor From Fermentation Broth Via Photodegradation and Macroporous Resin Adsorption

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 May 31

PMID 34054766

Citations 2

Authors

Bao Tang

Lingtian Wu

Jinzi Wang

Weibo Sun

Yancun Zhao

Fengquan Liu

Affiliations

Soon will be listed here.

Abstract

Heat-stable antifungal factor (HSAF) is produced by the fermentation of , which is known for its broad-spectrum antifungal activity and novel mode of action. However, studies on the separation of HSAF have rarely been reported. Herein, alteramide B (the main byproduct) was removed firstly from the fermentation broth by photodegradation to improve the purity of HSAF. Then, the separation of HSAF via adsorption by macroporous adsorption resins (MARs) was evaluated and NKA resin showed highest static adsorption and desorption performances. After optimizing the static and dynamic adsorption characteristics, the content of HSAF in the purified product increased from 8.67 ± 0.32% (ethyl acetate extraction) to 31.07 ± 1.12% by 3.58-fold. These results suggest that the developed strategy via photodegradation and macroporous resin adsorption is an effective process for the separation of HSAF, and it is also a promising method for the large-scale preparation of HSAF for agricultural applications.

Citing Articles

A novel and high-efficient method for the preparation of heat-stable antifungal factor from by high-speed counter-current chromatography.

Sun W, Tang B, Dong L, Xu J, Zhao Y, Liu F Front Microbiol. 2023; 14:1227244.

PMID: 37645219 PMC: 10461446. DOI: 10.3389/fmicb.2023.1227244.

Evaluating the Mode of Antifungal Action of Heat-Stable Antifungal Factor (HSAF) in .

Liu X, Jiang X, Sun H, Du J, Luo Y, Huang J J Fungi (Basel). 2022; 8(3).

PMID: 35330254 PMC: 8951606. DOI: 10.3390/jof8030252.

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