Expression of the MiR-302/367 MicroRNA Cluster is Regulated by a Conserved Long Non-coding Host-gene

Overview

Journal Sci Rep

Specialty Science

Date 2021 May 28

PMID 34045480

Citations 6

Authors

Karim Rahimi

Annette Christine Fuchtbauer

Fardin Fathi

Seyed Javad Mowla

Ernst-Martin Fuchtbauer

Affiliations

Soon will be listed here.

Abstract

MicroRNAs are important regulators of cellular functions. MiR-302/367 is a polycistronic miRNA cluster that can induce and maintain pluripotency. Here we investigate the transcriptional control and the processing of the miR-302 host-gene in mice. Our results indicate that the mmu-miR-302 host-gene is alternatively spliced, polyadenylated and exported from the nucleus. The regulatory sequences extend at least 2 kb upstream of the transcription start site and contain several conserved binding sites for both transcriptional activators and repressors. The gene structure and regulatory elements are highly conserved between mouse and human. So far, regulating miR-302 expression is the only known function of the miR-302 host-gene. Even though we here only provide one example, regulation of microRNA transcription might be a so far little recognized function of long non-coding RNA genes.

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References

Laird P, Zijderveld A, Linders K, Rudnicki M, Jaenisch R, Berns A . Simplified mammalian DNA isolation procedure. Nucleic Acids Res. 1991; 19(15):4293. PMC: 328579. DOI: 10.1093/nar/19.15.4293. View

Rahimi K, Parsa S, Nikzaban M, Khaledian B, Mowla S, Fathi F . Evaluation of miR-302 promoter activity in transgenic mice and pluripotent stem cell lines. In Vitro Cell Dev Biol Anim. 2020; 56(10):896-905. DOI: 10.1007/s11626-020-00516-8. View

Barroso-Del Jesus A, Lucena-Aguilar G, Menendez P . The miR-302-367 cluster as a potential stemness regulator in ESCs. Cell Cycle. 2009; 8(3):394-8. DOI: 10.4161/cc.8.3.7554. View

Hones J, Thivakaran A, Botezatu L, Patnana P, Vitoriano da Conceicao Castro S, Al-Matary Y . Enforced GFI1 expression impedes human and murine leukemic cell growth. Sci Rep. 2017; 7(1):15720. PMC: 5691148. DOI: 10.1038/s41598-017-15866-9. View

Zhu Z, Xu Y, Zhao J, Liu Q, Feng W, Fan J . miR-367 promotes epithelial-to-mesenchymal transition and invasion of pancreatic ductal adenocarcinoma cells by targeting the Smad7-TGF-β signalling pathway. Br J Cancer. 2015; 112(8):1367-75. PMC: 4402451. DOI: 10.1038/bjc.2015.102. View

Anokye-Danso F, Trivedi C, Juhr D, Gupta M, Cui Z, Tian Y . Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency. Cell Stem Cell. 2011; 8(4):376-88. PMC: 3090650. DOI: 10.1016/j.stem.2011.03.001. View

Greer Card D, Hebbar P, Li L, Trotter K, Komatsu Y, Mishina Y . Oct4/Sox2-regulated miR-302 targets cyclin D1 in human embryonic stem cells. Mol Cell Biol. 2008; 28(20):6426-38. PMC: 2577422. DOI: 10.1128/MCB.00359-08. View

Tian Y, Zhang Y, Hurd L, Hannenhalli S, Liu F, Lu M . Regulation of lung endoderm progenitor cell behavior by miR302/367. Development. 2011; 138(7):1235-45. PMC: 3050657. DOI: 10.1242/dev.061762. View

Borchert G, Lanier W, Davidson B . RNA polymerase III transcribes human microRNAs. Nat Struct Mol Biol. 2006; 13(12):1097-101. DOI: 10.1038/nsmb1167. View

10.

Lin S, Ying S . Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR-302 Induction. Methods Mol Biol. 2018; 1733:265-282. DOI: 10.1007/978-1-4939-7601-0_22. View

11.

Yee A, Paulson E, McDevitt M, Rieger-Christ K, Summerhayes I, Berasi S . The HBP1 transcriptional repressor and the p38 MAP kinase: unlikely partners in G1 regulation and tumor suppression. Gene. 2004; 336(1):1-13. DOI: 10.1016/j.gene.2004.04.004. View

12.

Parchem R, Ye J, Judson R, LaRussa M, Krishnakumar R, Blelloch A . Two miRNA clusters reveal alternative paths in late-stage reprogramming. Cell Stem Cell. 2014; 14(5):617-31. PMC: 4305531. DOI: 10.1016/j.stem.2014.01.021. View

13.

Moreno-Moya J, Vilella F, Simon C . MicroRNA: key gene expression regulators. Fertil Steril. 2013; 101(6):1516-23. DOI: 10.1016/j.fertnstert.2013.10.042. View

14.

Mapendano C, Lykke-Andersen S, Kjems J, Bertrand E, Jensen T . Crosstalk between mRNA 3' end processing and transcription initiation. Mol Cell. 2010; 40(3):410-22. DOI: 10.1016/j.molcel.2010.10.012. View

15.

Matys V, Kel-Margoulis O, Fricke E, Liebich I, Land S, Barre-Dirrie A . TRANSFAC and its module TRANSCompel: transcriptional gene regulation in eukaryotes. Nucleic Acids Res. 2005; 34(Database issue):D108-10. PMC: 1347505. DOI: 10.1093/nar/gkj143. View

16.

Rosa A, Brivanlou A . A regulatory circuitry comprised of miR-302 and the transcription factors OCT4 and NR2F2 regulates human embryonic stem cell differentiation. EMBO J. 2010; 30(2):237-48. PMC: 3025464. DOI: 10.1038/emboj.2010.319. View

17.

Sampson E, Haque Z, Ku M, Tevosian S, Albanese C, Pestell R . Negative regulation of the Wnt-beta-catenin pathway by the transcriptional repressor HBP1. EMBO J. 2001; 20(16):4500-11. PMC: 125566. DOI: 10.1093/emboj/20.16.4500. View

18.

Lee N, Kim J, Cho W, Lee M, Steiner R, Gompers A . miR-302b maintains "stemness" of human embryonal carcinoma cells by post-transcriptional regulation of Cyclin D2 expression. Biochem Biophys Res Commun. 2008; 377(2):434-440. DOI: 10.1016/j.bbrc.2008.09.159. View

19.

Balzano F, Cruciani S, Basoli V, Santaniello S, Facchin F, Ventura C . MiR200 and miR302: Two Big Families Influencing Stem Cell Behavior. Molecules. 2018; 23(2). PMC: 6017081. DOI: 10.3390/molecules23020282. View

20.

Swiatek P, Gridley T . Perinatal lethality and defects in hindbrain development in mice homozygous for a targeted mutation of the zinc finger gene Krox20. Genes Dev. 1993; 7(11):2071-84. DOI: 10.1101/gad.7.11.2071. View