Dengue 2 Virus Envelope Protein Expressed by a Recombinant Vaccinia Virus Fails to Protect Monkeys Against Dengue

Overview

Journal J Gen Virol

Specialty Microbiology

Date 1988 Aug 1

PMID 3404119

Citations 14

Authors

V Deubel

R M Kinney

J J Esposito

C B Cropp

A V Vorndam

T P Monath

D W TRENT

Affiliations

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Abstract

A cDNA copy of the dengue (DEN) 2 virus genome region encoding the virion capsid, membrane and envelope structural proteins has been inserted into vaccinia virus (VV) DNA under the control of its 11K late promoter. The DEN-2 envelope protein was expressed and processed in cells infected with the VV recombinant (VV/D2S). No DEN-2 virus antibody response was detected in mice, hamsters or monkeys vaccinated with VV/D2S. Furthermore, a viraemia was observed in recombinant-vaccinated monkeys after challenge with infectious DEN-2 virus.

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