Differential Regulation of Synthesis of Multiple Forms of Specific Endoglucanases by Trichoderma Reesei QM9414

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1988 Aug 1

PMID 3403510

Citations 5

Authors

R Messner

F Gruber

C P Kubicek

Affiliations

Soon will be listed here.

Abstract

A method consisting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent detection of endoglucanases by blotting with a polyclonal antibody against endoglucanase I was used to investigate the effect of induction and carbon catabolite derepression on the synthesis of multiple forms of endoglucanase I by Trichoderma reesei. Five forms appeared upon growth on cellulose, whereas four and only two appeared upon growth on lactose (carbon catabolite derepression) and induction by sophorose in a resting cell system, respectively. All endoglucanases detected resembled endoglucanase I in their specificity, since they exhibited no activity toward xylan or paranitrophenyl-beta-D-lactobioside. A small (25-kilodalton) endoglucanase only appeared during growth on cellulose. None of the multiple forms arose by postsecretional modification. The results indicate that sophorose may not be the only compound mediating cellulose induction of the specific endoglucanases in T. reesei.

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References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Mandels M, Parrish F, REESE E . Sophorose as an inducer of cellulase in Trichoderma viride. J Bacteriol. 1962; 83:400-8. PMC: 277742. DOI: 10.1128/jb.83.2.400-408.1962. View

Nisizawa T, Suzuki H, Nisizawa K . Catabolite repression of cellulase formation in Trichoderma viride. J Biochem. 1972; 71(6):999-1007. DOI: 10.1093/oxfordjournals.jbchem.a129872. View

Nakayama M, Tomita Y, Suzuki H, Nisizawa K . Partial proteolysis of some cellulase components from Trichoderma viride and the substrate specificity of the modified products. J Biochem. 1976; 79(5):955-66. DOI: 10.1093/oxfordjournals.jbchem.a131163. View

Shoemaker S, Brown Jr R . Characterization of endo-1,4-beta-D-glucanases purified from Trichoderma viride. Biochim Biophys Acta. 1978; 523(1):147-61. DOI: 10.1016/0005-2744(78)90017-7. View

Hakansson U, Fagerstam L, Pettersson G, Andersson L . Purification and characterization of a low molecular weight 1,4-beta-glucan glucanohydrolase from the cellulolytic fungus Trichoderma viride QM 9414. Biochim Biophys Acta. 1978; 524(2):385-92. DOI: 10.1016/0005-2744(78)90175-4. View

Hakansson U, Fagerstam L, Pettersson L, Andersson L . A 1,4-beta-glucan glucanohydrolase from the cellulolytic fungus Trichoderma viride QM 9414. Purification, characterization and preparation of an immunoadsorbent for the enzyme. Biochem J. 1979; 179(1):141-9. PMC: 1186604. DOI: 10.1042/bj1790141. View

Sternberg D, Mandels G . Induction of cellulolytic enzymes in Trichoderma reesei by sophorose. J Bacteriol. 1979; 139(3):761-9. PMC: 218020. DOI: 10.1128/jb.139.3.761-769.1979. View

Montenecourt B, Nhlapo S, Cuskey S, Schamhart D, Eveleigh D . Regulatory controls in relation to over-production of fungal cellulases. Basic Life Sci. 1981; 18:33-53. DOI: 10.1007/978-1-4684-3980-9_4. View

10.

Shoemaker S, Raymond J, Bruner R . Cellulases: diversity amongst improved Trichoderma strains. Basic Life Sci. 1981; 18:89-109. DOI: 10.1007/978-1-4684-3980-9_7. View

11.

Beldman G, Searle-Van Leeuwen M, Rombouts F, Voragen F . The cellulase of Trichoderma viride. Purification, characterization and comparison of all detectable endoglucanases, exoglucanases and beta-glucosidases. Eur J Biochem. 1985; 146(2):301-8. DOI: 10.1111/j.1432-1033.1985.tb08653.x. View

12.

Biely P, Markovic O, Mislovicova D . Sensitive detection of endo-1,4-beta-glucanases and endo-1,4-beta-xylanases in gels. Anal Biochem. 1985; 144(1):147-51. DOI: 10.1016/0003-2697(85)90096-x. View

13.

Merivuori H, Siegler K, Sands J, Montenecourt B . Regulation of cellulase biosynthesis and secretion in fungi. Biochem Soc Trans. 1985; 13(2):411-4. DOI: 10.1042/bst0130411. View

14.

Niku-Paavola M, Lappalainen A, Enari T, NUMMI M . A new appraisal of the endoglucanases of the fungus Trichoderma reesei. Biochem J. 1985; 231(1):75-81. PMC: 1152705. DOI: 10.1042/bj2310075. View

15.

Glenn M, Ghosh A, Ghosh B . Subcellular fractionation of a hypercellulolytic mutant, Trichoderma reesei Rut-C30: localization of endoglucanase in microsomal fraction. Appl Environ Microbiol. 1985; 50(5):1137-43. PMC: 238713. DOI: 10.1128/aem.50.5.1137-1143.1985. View

16.

Penttila M, Lehtovaara P, Nevalainen H, Bhikhabhai R, Knowles J . Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene. Gene. 1986; 45(3):253-63. DOI: 10.1016/0378-1119(86)90023-5. View

17.

Kubicek C . Involvement of a conidial endoglucanase and a plasma-membrane-bound beta-glucosidase in the induction of endoglucanase synthesis by cellulose in Trichoderma reesei. J Gen Microbiol. 1987; 133(6):1481-7. DOI: 10.1099/00221287-133-6-1481. View

18.

Nisizawa T, Suzuki H, Nakayama M, Nisizawa K . Inductive formation of cellulase by sophorose in Trichoderma viride. J Biochem. 1971; 70(3):375-85. DOI: 10.1093/oxfordjournals.jbchem.a129652. View