The Pluripotent Stem Cell-specific Transcript ESRG is Dispensable for Human Pluripotency

Overview

Journal PLoS Genet

Specialty Genetics

Date 2021 May 25

PMID 34033652

Citations 13

Authors

Kazutoshi Takahashi

Michiko Nakamura

Chikako Okubo

Zane Kliesmete

Mari Ohnuki

Megumi Narita

Akira Watanabe

Mai Ueda

Yasuhiro Takashima

Ines Hellmann

Shinya Yamanaka

Affiliations

Soon will be listed here.

Abstract

Human pluripotent stem cells (PSCs) express human endogenous retrovirus type-H (HERV-H), which exists as more than a thousand copies on the human genome and frequently produces chimeric transcripts as long-non-coding RNAs (lncRNAs) fused with downstream neighbor genes. Previous studies showed that HERV-H expression is required for the maintenance of PSC identity, and aberrant HERV-H expression attenuates neural differentiation potentials, however, little is known about the actual of function of HERV-H. In this study, we focused on ESRG, which is known as a PSC-related HERV-H-driven lncRNA. The global transcriptome data of various tissues and cell lines and quantitative expression analysis of PSCs showed that ESRG expression is much higher than other HERV-Hs and tightly silenced after differentiation. However, the loss of function by the complete excision of the entire ESRG gene body using a CRISPR/Cas9 platform revealed that ESRG is dispensable for the maintenance of the primed and naïve pluripotent states. The loss of ESRG hardly affected the global gene expression of PSCs or the differentiation potential toward trilineage. Differentiated cells derived from ESRG-deficient PSCs retained the potential to be reprogrammed into induced PSCs (iPSCs) by the forced expression of OCT3/4, SOX2, and KLF4. In conclusion, ESRG is dispensable for the maintenance and recapturing of human pluripotency.

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