Demonstration of Mononuclear Phagocytes in a Human Epiretinal Membrane Using a Monoclonal Anti-human Macrophage Antibody

Overview

Journal Graefes Arch Clin Exp Ophthalmol

Specialty Ophthalmology

Date 1988 Jan 1

PMID 3402749

Citations 9

Authors

M Weller

K Heimann

P Wiedemann

Affiliations

Soon will be listed here.

Abstract

Proliferative vitreoretinopathy (PVR), the major complication of rhegmatogenous retinal detachment and posterior segment ocular trauma, is a multistage disease process eventually resulting in traction retinal detachment. The migration of macrophages to a site of disrupted barriers between the vitreous, retina, and choroidea, respectively, is considered to be an important step in the early pathogenesis of PVR. In this study, we demonstrate the presence of numerous mononuclear phagocytes in a human PVR membrane by an APAAP (alkaline phosphatase-anti-alkaline phosphatase) immunostain using a monoclonal mouse anti-human macrophage antibody. This finding correlates with a highly positive fibronectin immunostain in the same specimen. Our results warrant further investigation of the involvement of mononuclear phagocytes in vitreoretinal proliferative disorders.

Citing Articles

Macrophages in proliferative vitreoretinopathy and proliferative diabetic retinopathy: differentiation of subpopulations.

Esser P, Heimann K, Wiedemann P Br J Ophthalmol. 1993; 77(11):731-3.

PMID: 8280690 PMC: 504635. DOI: 10.1136/bjo.77.11.731.

HLA antigens and lymphocytes in proliferative vitreoretinopathy.

Proenca R, Carvalho M, Proenca D, Verissimo J, Regadas I, Travassos A Graefes Arch Clin Exp Ophthalmol. 1994; 232(1):25-32.

PMID: 8119598 DOI: 10.1007/BF00176434.

Morphological and pathogenetic aspects of proliferative vitreo-retinopathy. A histological and immunohistochemical study.

Toti P, Greco G, Catella A Doc Ophthalmol. 1994; 88(2):105-12.

PMID: 7781478 DOI: 10.1007/BF01204608.

Immunohistochemistry of anterior proliferative vitreoretinopathy. Report of 11 cases.

Stodtler M, Mietz H, Wiedemann P, Heimann K Int Ophthalmol. 1994; 18(6):323-8.

PMID: 7642332 DOI: 10.1007/BF00930309.

The significance of vitronectin in proliferative diabetic retinopathy.

Esser P, Bresgen M, Weller M, Heimann K, Wiedemann P Graefes Arch Clin Exp Ophthalmol. 1994; 232(8):477-81.

PMID: 7523258 DOI: 10.1007/BF00195357.

References

MACHEMER R, Laqua H . Pigment epithelium proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol. 1975; 80(1):1-23. DOI: 10.1016/0002-9394(75)90862-4. View

Kampik A, Kenyon K, Michels R, Green W, De La Cruz Z . Epiretinal and vitreous membranes. Comparative study of 56 cases. Arch Ophthalmol. 1981; 99(8):1445-54. DOI: 10.1001/archopht.1981.03930020319025. View

Pollack A, Korte G, Heriot W, Henkind P . Ultrastructure of Bruch's membrane after krypton laser photocoagulation. II. Repair of Bruch's membrane and the role of macrophages. Arch Ophthalmol. 1986; 104(9):1377-82. DOI: 10.1001/archopht.1986.01050210131040. View

Campochiaro P, Jerdan J, Glaser B, CARDIN A, Michels R . Vitreous aspirates from patients with proliferative vitreoretinopathy stimulate retinal pigment epithelial cell migration. Arch Ophthalmol. 1985; 103(9):1403-5. DOI: 10.1001/archopht.1985.01050090155053. View

Leibovich S, Ross R . The role of the macrophage in wound repair. A study with hydrocortisone and antimacrophage serum. Am J Pathol. 1975; 78(1):71-100. PMC: 1915032. View

Gloor B . On the question of the origin of macrophages in the retina and the vitreous following photocoagulation (autoradiographic investigations by means of 3H-thymidine). Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1974; 190(3):183-94. DOI: 10.1007/BF00407092. View

Wiedemann P, Lemmen K, Schmiedl R, Heimann K . Intraocular daunorubicin for the treatment and prophylaxis of traumatic proliferative vitreoretinopathy. Am J Ophthalmol. 1987; 104(1):10-4. DOI: 10.1016/0002-9394(87)90286-8. View

Alitalo K, Hovi T, Vaheri A . Fibronectin is produced by human macrophages. J Exp Med. 1980; 151(3):602-13. PMC: 2185817. DOI: 10.1084/jem.151.3.602. View

Yamada K, Akiyama S, Hasegawa T, Hasegawa E, Humphries M, Kennedy D . Recent advances in research on fibronectin and other cell attachment proteins. J Cell Biochem. 1985; 28(2):79-97. DOI: 10.1002/jcb.240280202. View

10.

Cordell J, Falini B, Erber W, Ghosh A, Abdulaziz Z, MacDonald S . Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984; 32(2):219-29. DOI: 10.1177/32.2.6198355. View

11.

Miller B, Miller H, Patterson R, Ryan S . Retinal wound healing. Cellular activity at the vitreoretinal interface. Arch Ophthalmol. 1986; 104(2):281-5. DOI: 10.1001/archopht.1986.01050140139037. View

12.

Weller M, Heimann K, Wiedemann P . Cytotoxic effects of daunomycin on retinal pigment epithelium in vitro. Graefes Arch Clin Exp Ophthalmol. 1987; 225(3):235-8. DOI: 10.1007/BF02175455. View

13.

Marquette D, Molnar J, Yamada K, Schlesinger D, Darby S, Van Alten P . Phagocytosis-promoting activity of avian plasma and fibroblastic cell surface fibronectins. Mol Cell Biochem. 1981; 36(3):147-55. DOI: 10.1007/BF02357031. View

14.

Freundlich B, Avdalovic N . Use of gelatin/plasma coated flasks for isolating human peripheral blood monocytes. J Immunol Methods. 1983; 62(1):31-7. DOI: 10.1016/0022-1759(83)90107-2. View

15.

Hiscott P, Grierson I, McLeod D . Natural history of fibrocellular epiretinal membranes: a quantitative, autoradiographic, and immunohistochemical study. Br J Ophthalmol. 1985; 69(11):810-23. PMC: 1040751. DOI: 10.1136/bjo.69.11.810. View