A Eutherian-specific MicroRNA Controls the Translation of Satb2 in a Model of Cortical Differentiation

Overview

Journal Stem Cell Reports

Publisher Cell Press

Specialty Cell Biology

Date 2021 May 21

PMID 34019815

Citations 6

Authors

Manuella Martins

Silvia Galfre

Marco Terrigno

Luca Pandolfini

Irene Appolloni

Keagan Dunville

Andrea Marranci

Milena Rizzo

Alberto Mercatanti

Laura Poliseno

Francesco Morandin

Marco Pietrosanto

Manuela Helmer-Citterich

Paolo Malatesta

Robert Vignali

Federico Cremisi

Affiliations

Soon will be listed here.

Abstract

Cerebral cortical development is controlled by key transcription factors that specify the neuronal identities in the different layers. The mechanisms controlling their expression in distinct cells are only partially known. We investigated the expression and stability of Tbr1, Bcl11b, Fezf2, Satb2, and Cux1 mRNAs in single developing mouse cortical cells. We observe that Satb2 mRNA appears much earlier than its protein and in a set of cells broader than expected, suggesting an initial inhibition of its translation, subsequently released during development. Mechanistically, Satb2 3'UTR modulates protein translation of GFP reporters during mouse corticogenesis. We select miR-541, a eutherian-specific miRNA, and miR-92a/b as the best candidates responsible for SATB2 inhibition, being strongly expressed in early and reduced in late progenitor cells. Their inactivation triggers robust and premature SATB2 translation in both mouse and human cortical cells. Our findings indicate RNA interference as a major mechanism in timing cortical cell identities.

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