Determination of Serum Oxidative Stress, Antioxidant Capacity and Protein Profiles in Dogs Naturally Infected with Ehrlichia Canis

Overview

Journal Acta Parasitol

Specialty Parasitology

Date 2021 May 21

PMID 34018150

Citations 5

Authors

Gulay Ciftci

Didem Pekmezci

Murat Guzel

Sena Cenesiz

Kerem Ural

Nuran Aysul

Filiz Kazak

Affiliations

Soon will be listed here.

Abstract

Purpose: Canine ehrlichiosis is an important tick-borne disease of dogs worldwide. In the present study, we aimed to determine the serum total antioxidant capacity (TAC), malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase, (SOD), glutathione peroxidase (GSH-Px), adenosine deaminase (ADA) activity and serum protein profiles in dogs affected with naturally acquired ehrlichiosis.

Methods: The animal materials had been consisted of ten dogs naturally infected with Ehrlichia canis, and ten controls negative for Ehrlichia canis. TAC, MDA, NO, SOD, GSH-Px, ADA activity and TP, ALB, GLOB levels were measured in sera of the animals. The serum protein concentrations were measured by autoanalyzer. The electrophoretic profiles of serum total protein were determined by native polyacrylamide gel electrophoresis (Native-PAGE).

Results: In dogs with ehrlichiosis, decreased TAC (P < 0.05) and GSH-Px (P > 0.05) levels were determined. However, NO (P > 0.05), SOD (P < 0.05), ADA (P > 0.05), MDA (P > 0.05), TP (P < 0.05) and GLO (P < 0.05) levels were found as increased in the Ehrlichia positive dogs. ALB levels were decreased without a statistical significance (P > 0.05). ALB, α1 and β2 globulin strip densities were found as decreased in native-PAGE, while β1 and γ globulin strip densities were significantly increased in the E. canis positive group when compared to the control.

Conclusion: It was determined that the oxidative stress decreased high antioxidant activity in dogs naturally infected with E. canis, and consequently, pro-oxidant and antioxidant defense and serum protein profiles were affected. It was thought that antioxidant supplementation could be beneficial to the treatment of the disease.

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References

Zweygarth E, Cabezas-Cruz A, Josemans A, Oosthuizen M, Matjila P, Lis K . In vitro culture and structural differences in the major immunoreactive protein gp36 of geographically distant Ehrlichia canis isolates. Ticks Tick Borne Dis. 2014; 5(4):423-31. DOI: 10.1016/j.ttbdis.2014.01.011. View

Mylonakis M, Koutinas A, Breitschwerdt E, Hegarty B, Billinis C, Leontides L . Chronic canine ehrlichiosis (Ehrlichia canis): a retrospective study of 19 natural cases. J Am Anim Hosp Assoc. 2004; 40(3):174-84. DOI: 10.5326/0400174. View

Harrus S, Waner T, Avidar Y, Bogin E, Peh H, Bark H . Serum protein alterations in canine ehrlichiosis. Vet Parasitol. 1996; 66(3-4):241-9. DOI: 10.1016/s0304-4017(96)01013-8. View

Lykkesfeldt J, Svendsen O . Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet J. 2006; 173(3):502-11. DOI: 10.1016/j.tvjl.2006.06.005. View

Gate L, Paul J, Ba G, Tew K, Tapiero H . Oxidative stress induced in pathologies: the role of antioxidants. Biomed Pharmacother. 1999; 53(4):169-80. DOI: 10.1016/S0753-3322(99)80086-9. View

Beckman J, Koppenol W . Nitric oxide, superoxide, and peroxynitrite: the good, the bad, and ugly. Am J Physiol. 1996; 271(5 Pt 1):C1424-37. DOI: 10.1152/ajpcell.1996.271.5.C1424. View

Ganheim C, Hulten C, Carlsson U, Kindahl H, Niskanen R, Waller K . The acute phase response in calves experimentally infected with bovine viral diarrhoea virus and/or Mannheimia haemolytica. J Vet Med B Infect Dis Vet Public Health. 2003; 50(4):183-90. DOI: 10.1046/j.1439-0450.2003.00658.x. View

da Silva A, Franca R, Costa M, Paim F, Pimentel V, Schmatz R . Adenosine levels in serum and adenosine deaminase activity in blood cells of dogs infected by Rangelia vitalii. J Parasitol. 2013; 99(6):1125-8. DOI: 10.1645/13-176.1. View

Jenkins M . Serum protein electrophoresis. Methods Mol Med. 2011; 27:11-9. DOI: 10.1385/1-59259-689-4:11. View

10.

Woolliams J, Wiener G, Anderson P, McMurray C . Variation in the activities of glutathione peroxidase and superoxide dismutase and in the concentration of copper in the blood in various breed crosses of sheep. Res Vet Sci. 1983; 34(3):253-6. View

11.

Paglia D, Valentine W . Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70(1):158-69. View

12.

Erel O . A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005; 38(12):1103-11. DOI: 10.1016/j.clinbiochem.2005.08.008. View

13.

Jentzsch A, Bachmann H, Furst P, Biesalski H . Improved analysis of malondialdehyde in human body fluids. Free Radic Biol Med. 1996; 20(2):251-6. DOI: 10.1016/0891-5849(95)02043-8. View

14.

Parish C, Marchalonis J . A simple and rapid acrylamide gel method for estimating the molecular weights of proteins and protein subunits. Anal Biochem. 1970; 34(2):436-50. DOI: 10.1016/0003-2697(70)90128-4. View

15.

Candiano G, Bruschi M, Musante L, Santucci L, Ghiggeri G, Carnemolla B . Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis. 2004; 25(9):1327-33. DOI: 10.1002/elps.200305844. View

16.

Kovacic P, Cooksy A . Iminium metabolite mechanism for nicotine toxicity and addiction: Oxidative stress and electron transfer. Med Hypotheses. 2004; 64(1):104-11. DOI: 10.1016/j.mehy.2004.03.048. View

17.

van Haaften R, Haenen G, Evelo C, Bast A . Effect of vitamin E on glutathione-dependent enzymes. Drug Metab Rev. 2003; 35(2-3):215-53. DOI: 10.1081/dmr-120024086. View

18.

Chaudhuri S, Varshney J, Patra R . Erythrocytic antioxidant defense, lipid peroxides level and blood iron, zinc and copper concentrations in dogs naturally infected with Babesia gibsoni. Res Vet Sci. 2008; 85(1):120-4. DOI: 10.1016/j.rvsc.2007.09.001. View

19.

Raes M, Michiels C, Remacle J . Comparative study of the enzymatic defense systems against oxygen-derived free radicals: the key role of glutathione peroxidase. Free Radic Biol Med. 1987; 3(1):3-7. DOI: 10.1016/0891-5849(87)90032-3. View

20.

Britti D, Sconza S, Morittu V, Santori D, Boari A . Superoxide dismutase and Glutathione peroxidase in the blood of dogs with Leishmaniasis. Vet Res Commun. 2008; 32 Suppl 1:S251-4. DOI: 10.1007/s11259-008-9121-3. View