Silencing LncRNA AK136714 Reduces Endothelial Cell Damage and Inhibits Atherosclerosis

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2021 May 20

PMID 34015766

Citations 9

Authors

Jing Bai

Jianxia Liu

Zexian Fu

Yuanyuan Feng

Bing Wang

Wenjuan Wu

Ruiying Zhang

Affiliations

Soon will be listed here.

Abstract

Atherosclerosis correlates with ischemic cardio-cerebrovascular diseases such as coronary heart disease. Long non-coding RNAs (lncRNAs) can promote atherosclerosis. We investigated the role of the lncRNA AK136714 in atherosclerosis. Compared with the healthy group, lncRNA AK136714 expression was elevated in the plaque and plasma of the atherosclerosis patients in a GEO dataset. AK136714 silencing inhibited atherosclerosis formation in ApoE-/- mice. AK136714 silencing also protected the endothelial barrier and inhibited endothelial cell inflammation. assays showed that knockdown of AK136714 suppressed the inflammatory response and apoptosis in human umbilical vein endothelial cells (HUVECs). Moreover, AK136714 was found to bind directly to HuR to increase the mRNA stability of TNF-α, IL-1β and IL-6 mRNAs. In addition, AK136714 promoted the transcription of Bim. This study expands our understanding of the role of lncRNA AK136714 in atherosclerosis and provides potential drug targets for the treatment of atherosclerosis.

Citing Articles

Increased caveolin 1 by human antigen R exacerbates -induced atherosclerosis by modulating oxidative stress and inflammatory responses.

Miao F, Lei Y, Guo Y, Ma Y, Zhang Y, Jia B Cytojournal. 2024; 21:42.

PMID: 39737126 PMC: 11683369. DOI: 10.25259/Cytojournal_76_2024.

Role of LncRNAs in the Pathogenesis of Coronary Artery Disease.

Wu X, Li J, Sun G, Yang J, Peng Y, Bai X Rev Cardiovasc Med. 2024; 24(4):96.

PMID: 39076276 PMC: 11273030. DOI: 10.31083/j.rcm2404096.

Roles of long noncoding RNAs in human inflammatory diseases.

Zhang Y, Liu H, Niu M, Wang Y, Xu R, Guo Y Cell Death Discov. 2024; 10(1):235.

PMID: 38750059 PMC: 11096177. DOI: 10.1038/s41420-024-02002-6.

LncRNAs as Regulators of Atherosclerotic Plaque Stability.

Petkovic A, Erceg S, Munjas J, Ninic A, Vladimirov S, Davidovic A Cells. 2023; 12(14).

PMID: 37508497 PMC: 10378138. DOI: 10.3390/cells12141832.

Noncoding RNAs in atherosclerosis: regulation and therapeutic potential.

Qi L, Xing J, Yuan Y, Lei M Mol Cell Biochem. 2023; 479(5):1279-1295.

PMID: 37418054 PMC: 11116212. DOI: 10.1007/s11010-023-04794-0.

References

Agarwal S, Avery C, Ballantyne C, Catellier D, Nambi V, Saunders J . Sources of variability in measurements of cardiac troponin T in a community-based sample: the atherosclerosis risk in communities study. Clin Chem. 2011; 57(6):891-7. PMC: 4928588. DOI: 10.1373/clinchem.2010.159350. View

Pan J . LncRNA H19 promotes atherosclerosis by regulating MAPK and NF-kB signaling pathway. Eur Rev Med Pharmacol Sci. 2017; 21(2):322-328. View

Ross R . Atherosclerosis is an inflammatory disease. Am Heart J. 1999; 138(5 Pt 2):S419-20. DOI: 10.1016/s0002-8703(99)70266-8. View

Tao K, Hu Z, Zhang Y, Jiang D, Cheng H . LncRNA CASC11 improves atherosclerosis by downregulating IL-9 and regulating vascular smooth muscle cell apoptosis and proliferation. Biosci Biotechnol Biochem. 2019; 83(7):1284-1288. DOI: 10.1080/09168451.2019.1597621. View

Stancel N, Chen C, Ke L, Chu C, Lu J, Sawamura T . Interplay between CRP, Atherogenic LDL, and LOX-1 and Its Potential Role in the Pathogenesis of Atherosclerosis. Clin Chem. 2015; 62(2):320-7. DOI: 10.1373/clinchem.2015.243923. View

Deguchi J, Aikawa M, Tung C, Aikawa E, Kim D, Ntziachristos V . Inflammation in atherosclerosis: visualizing matrix metalloproteinase action in macrophages in vivo. Circulation. 2006; 114(1):55-62. DOI: 10.1161/CIRCULATIONAHA.106.619056. View

Miteva K, Madonna R, De Caterina R, Van Linthout S . Innate and adaptive immunity in atherosclerosis. Vascul Pharmacol. 2018; . DOI: 10.1016/j.vph.2018.04.006. View

Liu Z, Zhang M, Zhou T, Shen Q, Qin X . Exendin-4 promotes the vascular smooth muscle cell re-differentiation through AMPK/SIRT1/FOXO3a signaling pathways. Atherosclerosis. 2018; 276:58-66. DOI: 10.1016/j.atherosclerosis.2018.07.016. View

Meng F, Yan J, Ma Q, Jiao Y, Han L, Xu J . Expression status and clinical significance of lncRNA in the progression of atherosclerosis. PeerJ. 2018; 6:e4246. PMC: 5775756. DOI: 10.7717/peerj.4246. View

10.

Hansson G . Inflammation, atherosclerosis, and coronary artery disease. N Engl J Med. 2005; 352(16):1685-95. DOI: 10.1056/NEJMra043430. View

11.

STARY H, Chandler A, Dinsmore R, Fuster V, Glagov S, INSULL Jr W . A definition of advanced types of atherosclerotic lesions and a histological classification of atherosclerosis. A report from the Committee on Vascular Lesions of the Council on Arteriosclerosis, American Heart Association. Circulation. 1995; 92(5):1355-74. DOI: 10.1161/01.cir.92.5.1355. View

12.

Olmos Y, Valle I, Borniquel S, Tierrez A, Soria E, Lamas S . Mutual dependence of Foxo3a and PGC-1alpha in the induction of oxidative stress genes. J Biol Chem. 2009; 284(21):14476-84. PMC: 2682896. DOI: 10.1074/jbc.M807397200. View

13.

Ross R . The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature. 1993; 362(6423):801-9. DOI: 10.1038/362801a0. View

14.

Chen C, Cheng G, Yang X, Li C, Shi R, Zhao N . Tanshinol suppresses endothelial cells apoptosis in mice with atherosclerosis via lncRNA TUG1 up-regulating the expression of miR-26a. Am J Transl Res. 2016; 8(7):2981-91. PMC: 4969434. View

15.

Yu H, Fellows A, Foote K, Yang Z, Figg N, Littlewood T . FOXO3a (Forkhead Transcription Factor O Subfamily Member 3a) Links Vascular Smooth Muscle Cell Apoptosis, Matrix Breakdown, Atherosclerosis, and Vascular Remodeling Through a Novel Pathway Involving MMP13 (Matrix Metalloproteinase 13). Arterioscler Thromb Vasc Biol. 2018; 38(3):555-565. PMC: 5828387. DOI: 10.1161/ATVBAHA.117.310502. View

16.

Bukowska H, Jastrzebska M, Chelstowski K, Naruszewicz M . Decrease in fibrinogen and LDL-cholesterol levels upon supplementation of diet with Lactobacillus plantarum in subjects with moderately elevated cholesterol. Atherosclerosis. 1998; 137(2):437-8. DOI: 10.1016/s0021-9150(97)00283-9. View

17.

Yu H, Clarke M, Figg N, Littlewood T, Bennett M . Smooth muscle cell apoptosis promotes vessel remodeling and repair via activation of cell migration, proliferation, and collagen synthesis. Arterioscler Thromb Vasc Biol. 2011; 31(11):2402-9. DOI: 10.1161/ATVBAHA.111.235622. View

18.

Kurdi A, Martinet W, De Meyer G . mTOR Inhibition and Cardiovascular Diseases: Dyslipidemia and Atherosclerosis. Transplantation. 2017; 102(2S Suppl 1):S44-S46. DOI: 10.1097/TP.0000000000001693. View

19.

Yan B, Yao J, Liu J, Li X, Wang X, Li Y . lncRNA-MIAT regulates microvascular dysfunction by functioning as a competing endogenous RNA. Circ Res. 2015; 116(7):1143-56. DOI: 10.1161/CIRCRESAHA.116.305510. View

20.

Fu Y, Sun S, Sun H, Peng J, Ma X, Bao L . Scutellarin exerts protective effects against atherosclerosis in rats by regulating the Hippo-FOXO3A and PI3K/AKT signaling pathways. J Cell Physiol. 2019; 234(10):18131-18145. DOI: 10.1002/jcp.28446. View