Is Bioavailable Vitamin D Better Than Total Vitamin D to Evaluate Vitamin D Status in Obese Children?

Overview

Journal J Clin Res Pediatr Endocrinol

Specialties Endocrinology
Pediatrics

Date 2021 May 20

PMID 34013709

Citations 1

Authors

Gulin Karacan Kucukali

Ozlem Gulbahar

Servan Ozalkak

Hasan Dagli

Serdar Ceylaner

Zehra Aycan

Senay Savas Erdeve

Affiliations

Soon will be listed here.

Abstract

Introduction: Free hormones are biologically more active in target tissues. Thus, measurement of vitamin D taking into account bioavailability and free vitamin D may be preferable, especially when evidence is contradictory, as in obese children. In order to assess bioavailablity and free vitamin D, using a previously reported formula, vitamin D-binding protein (VDBP) level was measured and VDBP polymorphisms were also evaluated because of variations in binding affinity.

Methods: Eighty-four obese and 78 healthy children were included. Anthropometry, calcium, phosphorus, alkaline-phosphatase, parathyroid hormone (PTH), 25 hydroxyvitamin D [25(OH)D], bioavailable-free vitamin D, and VDBP concentration and polymorphism were evaluated in the whole group.

Results: Obese girls had significantly higher PTH than normal weight girls (p=0.001). Regardless of gender, obese children had significantly higher concentrations of VDBP (p=0.008) and PTH (p=0.002). When samples taken in winter were analyzed, PTH and VDBP were found to be higher and bioavailable and free vitamin D lower in the obese group. There was no difference in terms of total vitamin D between groups during the winter season.

Conclusion: While total, free, and bioavailable vitamin D in the obese group was similar to the control group in autumn, free and bioavailable vitamin D in the winter was lower in the obese than the control group. In addition, PTH was higher in the obese group in both autumn and winter. Therefore, more research is needed to evaluate the variability of free and bioavailable vitamin D according to body habitus, season and the effect any differences may have.

Citing Articles

Association of vitamin D levels with anthropometric and adiposity indicators across all age groups: a systematic review of epidemiologic studies.

Abiri B, Valizadeh M, Ahmadi A, Amini S, Nikoohemmat M, Abbaspour F Endocr Connect. 2023; 13(2).

PMID: 38032745 PMC: 10831555. DOI: 10.1530/EC-23-0394.

References

Hoofnagle A, Eckfeldt J, Lutsey P . Vitamin D-Binding Protein Concentrations Quantified by Mass Spectrometry. N Engl J Med. 2015; 373(15):1480-2. PMC: 4654614. DOI: 10.1056/NEJMc1502602. View

Chun R, Shieh A, Gottlieb C, Yacoubian V, Wang J, Hewison M . Vitamin D Binding Protein and the Biological Activity of Vitamin D. Front Endocrinol (Lausanne). 2019; 10:718. PMC: 6821678. DOI: 10.3389/fendo.2019.00718. View

Powe C, Evans M, Wenger J, Zonderman A, Berg A, Nalls M . Vitamin D-binding protein and vitamin D status of black Americans and white Americans. N Engl J Med. 2013; 369(21):1991-2000. PMC: 4030388. DOI: 10.1056/NEJMoa1306357. View

Bikle D, Siiteri P, Ryzen E, Haddad J . Serum protein binding of 1,25-dihydroxyvitamin D: a reevaluation by direct measurement of free metabolite levels. J Clin Endocrinol Metab. 1985; 61(5):969-75. DOI: 10.1210/jcem-61-5-969. View

Naderpoor N, Shorakae S, Abell S, Mousa A, Joham A, Moran L . Bioavailable and free 25-hydroxyvitamin D and vitamin D binding protein in polycystic ovary syndrome: Relationships with obesity and insulin resistance. J Steroid Biochem Mol Biol. 2017; 177:209-215. DOI: 10.1016/j.jsbmb.2017.07.012. View

Speeckaert M, Taes Y, De Buyzere M, Christophe A, Kaufman J, Delanghe J . Investigation of the potential association of vitamin D binding protein with lipoproteins. Ann Clin Biochem. 2010; 47(Pt 2):143-50. DOI: 10.1258/acb.2009.009018. View

Karlsson T, Osmancevic A, Jansson N, Hulthen L, Holmang A, Larsson I . Increased vitamin D-binding protein and decreased free 25(OH)D in obese women of reproductive age. Eur J Nutr. 2013; 53(1):259-67. DOI: 10.1007/s00394-013-0524-8. View

Eroglu Altinova A, Ozkan C, Akturk M, Gulbahar O, Yalcin M, Cakir N . Vitamin D-binding protein and free vitamin D concentrations in acromegaly. Endocrine. 2015; 52(2):374-9. DOI: 10.1007/s12020-015-0789-1. View

Dura-Trave T, Gallinas-Victoriano F, Malumbres-Chacon M, Ahmed-Mohamed L, Chueca-Guindulain M, Berrade-Zubiri S . Are there any seasonal variations in 25-hydroxyvitamin D and parathyroid hormone serum levels in children and adolescents with severe obesity?. Eur J Pediatr. 2020; 180(4):1203-1210. DOI: 10.1007/s00431-020-03857-4. View

10.

Schwartz J, Gallagher J, Jorde R, Berg V, Walsh J, Eastell R . Determination of Free 25(OH)D Concentrations and Their Relationships to Total 25(OH)D in Multiple Clinical Populations. J Clin Endocrinol Metab. 2018; 103(9):3278-3288. PMC: 6126881. DOI: 10.1210/jc.2018-00295. View

11.

Drincic A, Armas L, Van Diest E, Heaney R . Volumetric dilution, rather than sequestration best explains the low vitamin D status of obesity. Obesity (Silver Spring). 2012; 20(7):1444-8. DOI: 10.1038/oby.2011.404. View

12.

Powe C, Ricciardi C, Berg A, Erdenesanaa D, Collerone G, Ankers E . Vitamin D-binding protein modifies the vitamin D-bone mineral density relationship. J Bone Miner Res. 2011; 26(7):1609-16. PMC: 3351032. DOI: 10.1002/jbmr.387. View

13.

Walsh J, Evans A, Bowles S, Naylor K, Jones K, Schoenmakers I . Free 25-hydroxyvitamin D is low in obesity, but there are no adverse associations with bone health. Am J Clin Nutr. 2016; 103(6):1465-71. DOI: 10.3945/ajcn.115.120139. View

14.

Shao B, Jiang S, Muyiduli X, Wang S, Mo M, Li M . Vitamin D pathway gene polymorphisms influenced vitamin D level among pregnant women. Clin Nutr. 2017; 37(6 Pt A):2230-2237. DOI: 10.1016/j.clnu.2017.10.024. View

15.

Lauridsen A, Vestergaard P, Hermann A, Brot C, Heickendorff L, Mosekilde L . Plasma concentrations of 25-hydroxy-vitamin D and 1,25-dihydroxy-vitamin D are related to the phenotype of Gc (vitamin D-binding protein): a cross-sectional study on 595 early postmenopausal women. Calcif Tissue Int. 2005; 77(1):15-22. DOI: 10.1007/s00223-004-0227-5. View

16.

Yao P, Sun L, Lu L, Ding H, Chen X, Tang L . Effects of Genetic and Nongenetic Factors on Total and Bioavailable 25(OH)D Responses to Vitamin D Supplementation. J Clin Endocrinol Metab. 2016; 102(1):100-110. DOI: 10.1210/jc.2016-2930. View

17.

Neyzi O, Bundak R, Gokcay G, Gunoz H, Furman A, Darendeliler F . Reference Values for Weight, Height, Head Circumference, and Body Mass Index in Turkish Children. J Clin Res Pediatr Endocrinol. 2016; 7(4):280-93. PMC: 4805217. DOI: 10.4274/jcrpe.2183. View

18.

Lee C, Young K, Norris J, Rotter J, Liu Y, Lorenzo C . Association of Directly Measured Plasma Free 25(OH)D With Insulin Sensitivity and Secretion: The IRAS Family Study. J Clin Endocrinol Metab. 2017; 102(8):2781-2788. PMC: 5546855. DOI: 10.1210/jc.2017-00039. View

19.

Bhan I, Powe C, Berg A, Ankers E, Wenger J, Karumanchi S . Bioavailable vitamin D is more tightly linked to mineral metabolism than total vitamin D in incident hemodialysis patients. Kidney Int. 2012; 82(1):84-9. PMC: 3376220. DOI: 10.1038/ki.2012.19. View

20.

Carpenter T, Zhang J, Parra E, Ellis B, Simpson C, Lee W . Vitamin D binding protein is a key determinant of 25-hydroxyvitamin D levels in infants and toddlers. J Bone Miner Res. 2012; 28(1):213-21. PMC: 3511814. DOI: 10.1002/jbmr.1735. View