Integrated Transcriptomics and Epigenomics Reveal Chamber-specific and Species-specific Characteristics of Human and Mouse Hearts

Overview

Journal PLoS Biol

Specialty Biology

Date 2021 May 18

PMID 34003819

Citations 10

Authors

Junpeng Gao

Yuxuan Zheng

Lin Li

Minjie Lu

Xiangjian Chen

Yu Wang

Yanna Li

Xiaomeng Liu

Yun Gao

Yunuo Mao

Peng Zhao

Jinan Zhang

Fuchou Tang

Lei Song

Lu Wen

Jizheng Wang

Affiliations

Soon will be listed here.

Abstract

DNA methylation, chromatin accessibility, and gene expression represent different levels information in biological process, but a comprehensive multiomics analysis of the mammalian heart is lacking. Here, we applied nucleosome occupancy and methylome sequencing, which detected DNA methylation and chromatin accessibility simultaneously, as well as RNA-seq, for multiomics analysis of the 4 chambers of adult and fetal human hearts, and adult mouse hearts. Our results showed conserved region-specific patterns in the mammalian heart at transcriptome and DNA methylation level. Adult and fetal human hearts showed distinct features in DNA methylome, chromatin accessibility, and transcriptome. Novel long noncoding RNAs were identified in the human heart, and the gene expression profiles of major cardiovascular diseases associated genes were displayed. Furthermore, cross-species comparisons revealed human-specific and mouse-specific differentially expressed genes between the atria and ventricles. We also reported the relationship among multiomics and found there was a bell-shaped relationship between gene-body methylation and expression in the human heart. In general, our study provided comprehensive spatiotemporal and evolutionary insights into the regulation of gene expression in the heart.

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