Mining of Linear B Cell Epitopes of SARS-CoV-2 ORF8 Protein from COVID-19 Patients

Overview

Journal Emerg Microbes Infect

Specialty Infectious Diseases

Date 2021 May 18

PMID 34003073

Citations 7

Authors

Xiaohui Wang

Joy-Yan Lam

Linlei Chen

Shannon Wing-Ngor Au

Kelvin K W To

Kwok-Yung Yuen

Kin-Hang Kok

Affiliations

Soon will be listed here.

Abstract

Given the on-going SARS-CoV-2 pandemic, identification of immunogenic targets against the viral protein will provide crucial advances towards the development of sensitive diagnostic tools and vaccination strategies. Our previous study has found that ORF8 protein of SARS-CoV-2 is highly immunogenic and shows high sensitivity in identifying COVID-19 disease. In this study, by employing overlapping linear peptides, we characterized the IgG immunodominant regions on SARS-CoV-2 ORF8 protein that are seropositive in the sera from SARS-CoV-2-infected patients. The major immunogenic epitopes are localized at (1) N-termini alpha helix, (2) the resides spanning beta 2 and 3 sheets, and (3) the loop between beta 4 and 5 sheets. Additionally, hamster model infected by SARS-CoV-2 further validates the seropositivity of the linear epitopes , demonstrating a potential application of the linear peptide-based immunization strategy. Taken together, identification and validation of these B-cell linear epitopes will provide insights into the design of serological diagnostics and peptide-based vaccination approach against this pandemic virus of high priority.

Citing Articles

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A survey of ORF8 sequence and immunoinformatics features during alpha, delta, and wild type peaks of the SARS-CoV-2 pandemic in Iran.

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