Longitudinal in Vivo Imaging of Acute Neuropathology in a Monkey Model of Ebola Virus Infection

Overview

Journal Nat Commun

Specialty Biology

Date 2021 May 18

PMID 34001896

Citations 9

Authors

William Schreiber-Stainthorp

Jeffrey Solomon

Ji Hyun Lee

Marcelo Castro

Swati Shah

Neysha Martinez-Orengo

Rebecca Reeder

Dragan Maric

Robin Gross

Jing Qin

Katie R Hagen

Reed F Johnson

Dima A Hammoud

Affiliations

Soon will be listed here.

Abstract

Ebola virus (EBOV) causes neurological symptoms yet its effects on the central nervous system (CNS) are not well-described. Here, we longitudinally assess the acute effects of EBOV on the brain, using quantitative MR-relaxometry, 18F-Fluorodeoxyglucose PET and immunohistochemistry in a monkey model. We report blood-brain barrier disruption, likely related to high cytokine levels and endothelial viral infection, with extravasation of fluid, Gadolinium-based contrast material and albumin into the extracellular space. Increased glucose metabolism is also present compared to the baseline, especially in the deep gray matter and brainstem. This regional hypermetabolism corresponds with mild neuroinflammation, sporadic neuronal infection and apoptosis, as well as increased GLUT3 expression, consistent with increased neuronal metabolic demands. Neuroimaging changes are associated with markers of disease progression including viral load and cytokine/chemokine levels. Our results provide insight into the pathophysiology of CNS involvement with EBOV and may help assess vaccine/treatment efficacy in real time.

Citing Articles

Virus-induced brain pathology and the neuroinflammation-inflammation continuum: the neurochemists view.

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The Promise of Molecular Imaging: Focus on Central Nervous System Infections.

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Magnetic Resonance Imaging for Monitoring of Hepatic Disease Induced by Ebola Virus: a Nonhuman Primate Proof-of-Concept Study.

Lee J, Calcagno C, Feuerstein I, Solomon J, Mani V, Huzella L Microbiol Spectr. 2023; 11(3):e0353822.

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