Terminal Epitope-Dependent Branch Preference of Siglecs Toward -Glycans

Overview

Journal Front Mol Biosci

Specialty Biology

Date 2021 May 17

PMID 33996901

Citations 4

Authors

Shuaishuai Wang

Congcong Chen

Minhui Guan

Ding Liu

Xiu-Feng Wan

Lei Li

Affiliations

Soon will be listed here.

Abstract

Siglecs are sialic acid-binding immunoglobulin-like lectins that play vital roles in immune cell signaling. Siglecs help the immune system distinguish between self and nonself through the recognition of glycan ligands. While the primary binding specificities of Siglecs are known to be divergent, their specificities for complex glycans remain unclear. Herein, we determined -glycan binding profiles of a set of Siglecs by using a complex asymmetric -glycan microarray. Our results showed that Siglecs had unique terminal epitope-dependent branch preference when recognizing asymmetric -glycans. Specifically, human Siglec-3, -9, and -10 prefer the α1-3 branch when Siaα2-6Galβ1-4GlcNAc terminal epitope serves as the binding ligand but prefer the opposite α1-6 branch when Siaα2-3Galβ1-4GlcNAc epitope serves as the ligand. Interestingly, Siglec-10 exhibited dramatic binding divergence toward a pair of Neu5Ac-containing asymmetric -glycan isomers, as well as their Neu5Gc-containing counterparts. This new information on complex glycan recognition by Siglecs provides insights into their biological roles and applications.

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