Structural Basis of RICs Iron Donation for Iron-Sulfur Cluster Biogenesis

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 May 17

PMID 33995335

Citations 2

Authors

Liliana S O Silva

Pedro M Matias

Celia V Romao

Ligia M Saraiva

Affiliations

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Abstract

YtfE is a di-iron protein of the widespread Repair of Iron Centers proteins (RIC) family that has the capacity to donate iron, which is a crucial component of the biogenesis of the ubiquitous family of iron-sulfur proteins. In this work we identify in a previously unrecognized link between the YtfE protein and the major bacterial system for iron-sulfur cluster (ISC) assembly. We show that YtfE establishes protein-protein interactions with the scaffold IscU, where the transient cluster is formed, and the cysteine desulfurase IscS. Moreover, we found that promotion by YtfE of the formation of an Fe-S cluster in IscU requires two glutamates, E125 and E159 in YtfE. Both glutamates form part of the entrance of a protein channel in YtfE that links the di-iron center to the surface. In particular, E125 is crucial for the exit of iron, as a single mutation to leucine closes the channel rendering YtfE inactive for the build-up of Fe-S clusters. Hence, we provide evidence for the key role of RICs as bacterial iron donor proteins involved in the biogenesis of Fe-S clusters.

Citing Articles

Repair of Iron Center Proteins-A Different Class of Hemerythrin-like Proteins.

Silva L, Matias P, Romao C, Saraiva L Molecules. 2022; 27(13).

PMID: 35807291 PMC: 9268430. DOI: 10.3390/molecules27134051.

The Di-Iron Protein YtfE Is a Nitric Oxide-Generating Nitrite Reductase Involved in the Management of Nitrosative Stress.

Crack J, Balasiny B, Bennett S, Rolfe M, Froes A, MacMillan F J Am Chem Soc. 2022; 144(16):7129-7145.

PMID: 35416044 PMC: 9052748. DOI: 10.1021/jacs.1c12407.

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