Glial Fibrillary Acidic Protein in Tumours of the Nervous System

Overview

Journal Br J Cancer

Specialty Oncology

Date 1978 Jan 1

PMID 339941

Citations 16

Authors

B Delpech

A DELPECH

M N Vidard

N Girard

J TAYOT

J C Clement

P Creissard

Affiliations

Soon will be listed here.

Abstract

Glial fibrillary acidic protein (GFA) was assayed in nerve-tumour extracts and located in these tumours by indirect immunofluorescence study. We conclude that GFA is a specific marker of both malignant and normal astrocytes. Non-astrocytic tumours (oligodendroglioma, meningioma) do not contain GFA. Tumours with astrocytic differentiation potential (medulloblastoma) may contain GFA. Comparison of microscopic and GFA assays leads us to conclude that GFA concentration is proportional to the amount of malignant astrocytes in the tumour and inversely proportional to the necrotic area of a tumour. Normal tissue GFA and glioblastoma GFA were found to be immunologically identical.

Citing Articles

Importance of GFAP isoform-specific analyses in astrocytoma.

van Bodegraven E, van Asperen J, Robe P, Hol E Glia. 2019; 67(8):1417-1433.

PMID: 30667110 PMC: 6617972. DOI: 10.1002/glia.23594.

Permanent increase of immunocytochemical reactivity for gamma-aminobutyric acid (GABA), glutamic acid decarboxylase, mitochondrial enzymes, and glial fibrillary acidic protein in rat cerebral cortex damaged by early postnatal hypoxia-ischemia.

Romijn H, Janszen A, Van den Bogert C Acta Neuropathol. 1994; 87(6):612-27.

PMID: 7522389 DOI: 10.1007/BF00293323.

Glial fibrillary acidic protein in medulloblastoma.

Mannoji H, Takeshita I, Fukui M, Ohta M, Kitamura K Acta Neuropathol. 1981; 55(1):63-9.

PMID: 7348008 DOI: 10.1007/BF00691533.

Immunobiologic aspects of the brain and human gliomas. A review.

Wikstrand C, Bigner D Am J Pathol. 1980; 98(2):517-68.

PMID: 6986786 PMC: 1903417.

Immunofluorescent staining of frozen sections for glial fibrillary acidic protein.

McKeever P, Laverson S, KORNBLITH P, Howard R, Quindlen E, Smith B Acta Neuropathol. 1982; 58(1):69-72.

PMID: 6753449 DOI: 10.1007/BF00692700.

References

Eng L, Vanderhaeghen J, Bignami A, Gerstl B . An acidic protein isolated from fibrous astrocytes. Brain Res. 1971; 28(2):351-4. DOI: 10.1016/0006-8993(71)90668-8. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Dittmann L, Axelsen N, Norgaard-Pedersen B, Bock E . Antigens in human glioblastomas and meningiomas: Search for tumour and onco-foetal antigens. Estimation of S-100 and GFA protein. Br J Cancer. 1977; 35(2):135-41. PMC: 2025329. DOI: 10.1038/bjc.1977.20. View

Delpech B, Vidard M, Schlosser M, Hernot C . [Various properties of an antigenically neurospecific glycoprotein]. C R Seances Soc Biol Fil. 1973; 167(6):1029-32. View

Avrameas S, Ternynck T . The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry. 1969; 6(1):53-66. DOI: 10.1016/0019-2791(69)90178-5. View

Delpech B, DELPECH A, Clement J, LAUMONIER R . [Immunochemical and immunological study of human brain tumors]. Int J Cancer. 1972; 9(2):374-82. DOI: 10.1002/ijc.2910090216. View

Maunoury R, DELPECH A, Delpech B, Vidard M, VEDRENNE C . Presence of neurospecific antigen NSA 1 in fetal human astrocytes in long-term culture. Brain Res. 1976; 112(2):383-7. DOI: 10.1016/0006-8993(76)90292-4. View

UYEDA C, Eng L, Bignami A . Immunological study of the glial fibrillary acidic protein. Brain Res. 1972; 37(1):81-9. DOI: 10.1016/0006-8993(72)90347-2. View

Delpech B, DELPECH A . [Immunochemical characterization of a neuro-specific, non species specific antigen. Quantitative study and histological localization in the rat]. Immunochemistry. 1975; 12(8):691-7. DOI: 10.1016/0019-2791(75)90217-7. View

10.

Bignami A, Dahl D . Differentiation of astrocytes in the cerebellar cortex and the pyramidal tracts of the newborn rat. An immunofluorescence study with antibodies to a protein specific to astrocytes. Brain Res. 1973; 49(2):393-402. DOI: 10.1016/0006-8993(73)90430-7. View

11.

Mancini G, Carbonara A, Heremans J . Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965; 2(3):235-54. DOI: 10.1016/0019-2791(65)90004-2. View

12.

WARECKA K . Immunochemical studies on a water-soluble-brain specific glycoprotein from human and rat brain. Life Sci. 1967; 6(18):1999-2002. DOI: 10.1016/0024-3205(67)90261-5. View

13.

Delpech B, BUFFE D . [Immunochemical study of saline extracts of human brain]. Ann Inst Pasteur (Paris). 1972; 122(2):331-40. View