Dorsal Raphe Serotonergic Neurons Promote Arousal from Isoflurane Anesthesia

Overview

Journal CNS Neurosci Ther

Specialties Neurology
Pharmacology

Date 2021 May 11

PMID 33973716

Citations 22

Authors

Ao Li

Rui Li

Pengrong Ouyang

Huihui Li

Sa Wang

Xinxin Zhang

Dan Wang

Mingzi Ran

Guangchao Zhao

Qianzi Yang

Zhenghua Zhu

Hailong Dong

Haopeng Zhang

Affiliations

Soon will be listed here.

Abstract

Aims: General anesthesia has been widely applied in surgical or nonsurgical medical procedures, but the mechanism behind remains elusive. Because of shared neural circuits of sleep and anesthesia, whether serotonergic system, which is highly implicated in modulation of sleep and wakefulness, regulates general anesthesia as well is worth investigating.

Methods: Immunostaining and fiber photometry were used to assess the neuronal activities. Electroencephalography spectra and burst-suppression ratio (BSR) were used to measure anesthetic depth and loss or recovery of righting reflex to indicate the induction or emergence time of general anesthesia. Regulation of serotonergic system was achieved through optogenetic, chemogenetic, or pharmacological methods.

Results: We found that both Fos expression and calcium activity were significantly decreased during general anesthesia. Activation of 5-HT neurons in the dorsal raphe nucleus (DRN) decreased the depth of anesthesia and facilitated the emergence from anesthesia, and inhibition deepened the anesthesia and prolonged the emergence time. Furthermore, agonism or antagonism of 5-HT 1A or 2C receptors mimicked the effect of manipulating DRN serotonergic neurons.

Conclusion: Our results demonstrate that 5-HT neurons in the DRN play a regulative role of general anesthesia, and activation of serotonergic neurons could facilitate emergence from general anesthesia partly through 5-HT 1A and 2C receptors.

Citing Articles

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Shared subcortical arousal systems across sensory modalities during transient modulation of attention.

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A hypothalamus-lateral periaqueductal gray GABAergic neural projection facilitates arousal following sevoflurane anesthesia in mice.

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Neural Network Mechanisms Underlying General Anesthesia: Cortical and Subcortical Nuclei.

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Myelin modulates the process of isoflurane anesthesia through the regulation of neural activity.

Wang X, Yi R, Liang X, Zhang N, Zhong F, Lu Y CNS Neurosci Ther. 2024; 30(8):e14922.

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