Signals Controlling Alternative Splicing of Major Histocompatibility Complex H-2 Class I Pre-mRNA

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1988 Jan 1

PMID 3397132

Citations 5

Authors

D E Handy

J McCluskey

A M Lew

J E Coligan

D H Margulies

Affiliations

Soon will be listed here.

Abstract

The use of alternative splice acceptor sites during the removal of intron 7 in pre-mRNA splicing produces two forms of H-2Kb protein: the predominant form, derived from a transcript that has spliced at the upstream splice acceptor site for exon 8 (long exon 8), and a Kb molecule derived from a transcript that has spliced at the downstream acceptor site for exon 8 (short exon 8). We have identified a potential lariat branch point adenosine for the upstream acceptor splice site. This adenosine is found 28 bp from the splice junction and is contained in the sequence AGTGATGG. D-region genes, which use only the downstream splice site, have the sequence AGTGGTGG. We have used in vitro mutagenesis to change this A of the H-2Kb gene to G and have made the reciprocal change in H-2Dd. Elimination of this adenosine in H-2Kb alters the pattern of pre-mRNA splicing and results in a predominance of the Kb molecules with short exon 8 encoded sequences. However, the addition of an adenosine in H-2Dd is not sufficient to direct splicing to the upstream site.

Citing Articles

Major histocompatibility complex (MHC) class I KbDb -/- deficient mice possess functional CD8+ T cells and natural killer cells.

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Characterization of a class Ib gene of the rat major histocompatibility complex.

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A negative regulatory element in the promoter region of the rat alpha 2A-adrenergic receptor gene overlaps an SP1 consensus binding site.

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Locus-specific cDNA cloning in the class I multigene family: structure of H-2Dr and H-2Ds.

Cai Z, Pease L Immunogenetics. 1990; 32(6):456-9.

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An intragenic recombinant class I gene: H-2Ddx.

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