First Detection and Complete Genome Analysis of the Lyon IARC Polyomavirus in China from Samples of Diarrheic Cats

Overview

Journal Virus Genes

Specialties Microbiology
Molecular Biology

Date 2021 May 10

PMID 33970402

Citations 4

Authors

Yuying Li

Haixin Huang

Tian Lan

Wei Wang

Jie Zhang

Min Zheng

Liang Cao

Wenchao Sun

Huijun Lu

Affiliations

Soon will be listed here.

Abstract

Lyon IARC polyomavirus (LIPyV), a newly discovered polyomavirus (PyV), was first identified in 2017 in human skin samples in the USA. Later, it was detected in several other countries in samples of human and feline origin. Our aim was to find out if the virus occurs in China. To this end, 100 fecal samples were collected from cats with diarrhea in Guangxi Province during 2016 and 2018 and tested with polymerase chain reaction (PCR). Only 2 samples that originated from two related individuals were found to be positive. Based on the sequence identity of the 240-bp PCR products, the two positive samples supposedly contained identical viruses. Therefore, only one of them, which was designated as LIPyV-GXNN01, was selected for full genome amplification, cloning, sequencing and analysis. LIPyV-GXNN01, which comprises 5,263 nucleotides, has an early region that consists of small T antigen (ST-Ag) and large T antigen (LT-Ag) and a late region coding for the VP1, VP2, and VP3 structural proteins. Moreover, the LIPyV-GXNN01 strain structural proteins share 95.9-99.4%, 97.6-99.2%, and 97.1-99.2% nucleic acid identity with the VP1, VP2, and VP3of other LIPyV reference strains, respectively. A phylogenetic analysis revealed that GXNN01 clustered together with previously reported LIPyV strain. This present study is the first report of LIPyV in China.

Citing Articles

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References

Araldi R, Assaf S, de Carvalho R, Carvalho M, Souza J, Magnelli R . Papillomaviruses: a systematic review. Genet Mol Biol. 2017; 40(1):1-21. PMC: 5409773. DOI: 10.1590/1678-4685-GMB-2016-0128. View

Szczerba-Turek A, Siemionek J, Ras A, Bancerz-Kisiel A, Platt-Samoraj A, Lipczynska-Ilczuk K . Genetic evaluation of bovine papillomavirus types detected in equine sarcoids in Poland. Pol J Vet Sci. 2019; 22(1):25-29. DOI: 10.24425/pjvs.2018.125602. View

Crespo S, Lunardi M, Otonel R, Headley S, Alfieri A, Alfieri A . Genetic characterization of a putative new type of bovine papillomavirus in the Xipapillomavirus 1 species in a Brazilian dairy herd. Virus Genes. 2019; 55(5):682-687. DOI: 10.1007/s11262-019-01694-8. View

Lopez-Bueno A, Mavian C, Labella A, Castro D, Borrego J, Alcami A . Concurrence of Iridovirus, Polyomavirus, and a Unique Member of a New Group of Fish Papillomaviruses in Lymphocystis Disease-Affected Gilthead Sea Bream. J Virol. 2016; 90(19):8768-79. PMC: 5021401. DOI: 10.1128/JVI.01369-16. View

Dong J, Zhu W, Yamashita N, Chambers J, Uchida K, Kuwano A . Isolation of equine papillomavirus type 1 from racing horse in Japan. J Vet Med Sci. 2017; 79(12):1957-1959. PMC: 5745171. DOI: 10.1292/jvms.17-0322. View

Haspeslagh M, Vlaminck L, Martens A . The possible role of Stomoxys calcitrans in equine sarcoid transmission. Vet J. 2018; 231:8-12. DOI: 10.1016/j.tvjl.2017.11.009. View

Stevens H, Rector A, Van Der Kroght K, Van Ranst M . Isolation and cloning of two variant papillomaviruses from domestic pigs: Sus scrofa papillomaviruses type 1 variants a and b. J Gen Virol. 2008; 89(Pt 10):2475-2481. DOI: 10.1099/vir.0.2008/003186-0. View

Link E, Hoferer M, Strobel B, Rigbers K, Langenmayer M, Sutter G . Sus scrofa papillomavirus 2 - genetic characterization of a novel suid papillomavirus from wild boar in Germany. J Gen Virol. 2017; 98(8):2113-2117. DOI: 10.1099/jgv.0.000868. View

Kamminga S, van der Meijden E, de Brouwer C, Feltkamp M, Zaaijer H . Prevalence of DNA of fourteen human polyomaviruses determined in blood donors. Transfusion. 2019; 59(12):3689-3697. PMC: 6916541. DOI: 10.1111/trf.15557. View

10.

Garcia-Perez R, Ibanez C, Godinez J, Arechiga N, Garin I, Perez-Suarez G . Novel papillomaviruses in free-ranging Iberian bats: no virus-host co-evolution, no strict host specificity, and hints for recombination. Genome Biol Evol. 2014; 6(1):94-104. PMC: 3914694. DOI: 10.1093/gbe/evt211. View

11.

Csoma E, Lengyel G, Banyai K, Takacs P, Anosi N, Marton S . Study of Karolinska Institutet and Washington University polyomaviruses in tonsil, adenoid, throat swab and middle ear fluid samples. Future Microbiol. 2018; 13:1719-1730. DOI: 10.2217/fmb-2018-0280. View

12.

Makiyama K, Hirai R, Matsuzaki H, Ikeda M . Assessment of human papilloma virus infection in adult laryngeal papilloma using a screening test. J Voice. 2013; 27(2):230-5. DOI: 10.1016/j.jvoice.2012.10.014. View

13.

Brostoff T, Dela Cruz Jr F, Church M, Woolard K, Pesavento P . The raccoon polyomavirus genome and tumor antigen transcription are stable and abundant in neuroglial tumors. J Virol. 2014; 88(21):12816-24. PMC: 4248892. DOI: 10.1128/JVI.01912-14. View

14.

Gheit T, Dutta S, Oliver J, Robitaille A, Hampras S, Combes J . Isolation and characterization of a novel putative human polyomavirus. Virology. 2017; 506:45-54. PMC: 9265179. DOI: 10.1016/j.virol.2017.03.007. View

15.

Kourieh A, Combes J, Tommasino M, Dalstein V, Clifford G, Guily J . Prevalence and risk factors of human polyomavirus infections in non-malignant tonsils and gargles: the SPLIT study. J Gen Virol. 2018; 99(12):1686-1698. DOI: 10.1099/jgv.0.001156. View

16.

Kamminga S, van der Meijden E, Wunderink H, Touze A, Zaaijer H, Feltkamp M . Development and Evaluation of a Broad Bead-Based Multiplex Immunoassay To Measure IgG Seroreactivity against Human Polyomaviruses. J Clin Microbiol. 2018; 56(4). PMC: 5869810. DOI: 10.1128/JCM.01566-17. View

17.

Fahsbender E, Altan E, Estrada M, Seguin M, Young P, Leutenegger C . Lyon-IARC Polyomavirus DNA in Feces of Diarrheic Cats. Microbiol Resour Announc. 2019; 8(29). PMC: 6639616. DOI: 10.1128/MRA.00550-19. View

18.

Leal E, Liang R, Liu Q, Villanova F, Shi L, Liang L . Regional adaptations and parallel mutations in Feline panleukopenia virus strains from China revealed by nearly-full length genome analysis. PLoS One. 2020; 15(1):e0227705. PMC: 6964837. DOI: 10.1371/journal.pone.0227705. View

19.

Zhang Q, Niu J, Yi S, Dong G, Yu D, Guo Y . Development and application of a multiplex PCR method for the simultaneous detection and differentiation of feline panleukopenia virus, feline bocavirus, and feline astrovirus. Arch Virol. 2019; 164(11):2761-2768. PMC: 7086731. DOI: 10.1007/s00705-019-04394-8. View

20.

Yi S, Niu J, Wang H, Dong G, Guo Y, Dong H . Molecular characterization of feline astrovirus in domestic cats from Northeast China. PLoS One. 2018; 13(10):e0205441. PMC: 6177177. DOI: 10.1371/journal.pone.0205441. View