Comparison of Cytokeratin Expressions Among Orthokeratinized Odontogenic Cysts, Epidermoid Cysts and Odontogenic Keratocysts: An Immunohistochemical Study

Overview

Journal J Oral Maxillofac Pathol

Date 2021 May 10

PMID 33967483

Citations 1

Authors

V M Padmapriya

B Kavitha

B Sivapathasundram

J Nagaraj

Affiliations

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Abstract

Background: Orthokeratinized odontogenic cysts are keratinizing jaw cysts and due their association with impacted teeth and keratinaceous content, they resemble odontogenic keratocysts but differ in regards to biological behaviour, being less aggressive. To unravel the nature of OOCs, as they resemble epidermoid cysts histologically and due to their developmental resemblances to OKCs, this study was conducted.

Aim And Objective: To compare the cytokeratin expressions of CK 10 and CK 19 among orthokeratinized odontogenic keratocysts, epidermoid cysts and odontogenic keratocysts by immunohistochemical study.

Materials And Methods: 30 cases of all three cysts were collected, 10 cases in each of these cysts (OOCs, EDCs and OKCs) were incubated with CK 10 and CK 19 markers respectively. IHC staining was performed and assessed all layers of epithelium. All the data were analyzed using SPSS software, P values were obtained by the Chi-square test and Fisher's test.

Results: The expression pattern of CK10 showed 100% positive in both OOCs and EDCs with significant difference in OKCs. CK19 expression, between EDCs and OKCs was significant but between OOCs & EDCs and OOCs & OKCs was found to be statistically insignificant.

Conclusion: CK 10 expressions in both OOCs and EDCs were near identical both in terms of expression and patterns of expression in surface and spinous layers. OOCs may not be distinguished from EDCs both histologically and with CK 10 expression. CK19 expression between OOCs & EDCs and OOCs & OKCs was statistically insignificant. Thus, based upon CK 19 expression, no significant differences were found between OOCs & EDCs and OOCs & OKCs, implying that OOCs resemble both EDCs and OKCs.

Citing Articles

Immunohistochemical Analysis of Dentigerous Cysts and Odontogenic Keratocysts Associated with Impacted Third Molars-A Systematic Review.

Almeida L, Loyd D, Boettcher D, Kraft O, Zammuto S Diagnostics (Basel). 2024; 14(12).

PMID: 38928661 PMC: 11202764. DOI: 10.3390/diagnostics14121246.

References

Hoshino M, Inoue H, Kikuchi K, Miyazaki Y, Yoshino A, Hara H . Comparative study of cytokeratin and langerin expression in keratinized cystic lesions of the oral and maxillofacial regions. J Oral Sci. 2015; 57(4):287-94. DOI: 10.2334/josnusd.57.287. View

Mirza S, Fadl S, Napaki S, Abualruz A . Case report of complicated epidermoid cyst of the floor of the mouth: Radiology-histopathology correlation. Qatar Med J. 2014; 2014(1):12-6. PMC: 4197368. DOI: 10.5339/qmj.2014.2. View

Maurette P, Jorge J, de Moraes M . Conservative treatment protocol of odontogenic keratocyst: a preliminary study. J Oral Maxillofac Surg. 2006; 64(3):379-83. DOI: 10.1016/j.joms.2005.11.007. View

Madras J, Lapointe H . Keratocystic odontogenic tumour: reclassification of the odontogenic keratocyst from cyst to tumour. J Can Dent Assoc. 2008; 74(2):165-165h. View

Imran A, Jayanthi P, Tanveer S, Gobu S . Classification of odontogenic cysts and tumors - Antecedents. J Oral Maxillofac Pathol. 2016; 20(2):269-71. PMC: 4989559. DOI: 10.4103/0973-029X.185935. View

Uppala D, Majumdar S, Rao K, Reddy S . Epidermoid cyst of the soft palate in an infant. J Oral Maxillofac Pathol. 2016; 19(3):409. PMC: 4774307. DOI: 10.4103/0973-029X.174685. View

Tsirevelou P, Papamanthos M, Chlopsidis P, Zourou I, Skoulakis C . Epidermoid cyst of the floor of the mouth: two case reports. Cases J. 2010; 2:9360. PMC: 2804007. DOI: 10.1186/1757-1626-2-9360. View

Walid M, Osborne T, Robinson J . Primary brain sarcoma or metastatic carcinoma?. Indian J Cancer. 2009; 46(2):174-5. DOI: 10.4103/0019-509x.49160. View

Moll R, Franke W, Schiller D, Geiger B, Krepler R . The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982; 31(1):11-24. DOI: 10.1016/0092-8674(82)90400-7. View

10.

Tsuji K, Wato M, Hayashi T, Yasuda N, Matsushita T, Ito T . The expression of cytokeratin in keratocystic odontogenic tumor, orthokeratinized odontogenic cyst, dentigerous cyst, radicular cyst and dermoid cyst. Med Mol Morphol. 2013; 47(3):156-61. DOI: 10.1007/s00795-013-0058-4. View

11.

Aragaki T, Michi Y, Katsube K, Uzawa N, Okada N, Akashi T . Comprehensive keratin profiling reveals different histopathogenesis of keratocystic odontogenic tumor and orthokeratinized odontogenic cyst. Hum Pathol. 2010; 41(12):1718-25. DOI: 10.1016/j.humpath.2010.05.007. View

12.

Bhakhar V, Shah V, Ghanchi M, Gosavi S, Srivastava H, Pachore N . A Comparative Analysis of Cytokeratin 18 and 19 Expressions in Odontogenic Keratocyst, Dentigerous Cyst and Radicular Cyst with a Review of Literature. J Clin Diagn Res. 2016; 10(7):ZC85-9. PMC: 5020168. DOI: 10.7860/JCDR/2016/20535.8206. View

13.

Selvamani M, Devi A, Basandi P, Madhushankari G . Prevalence and clinicopathological comparison of kerotocystic odontogenic tumor and orthokeratinized odontogenic cyst in South Indian sample population: A retrospective study over 13 years. J Pharm Bioallied Sci. 2014; 6(Suppl 1):S127-30. PMC: 4157249. DOI: 10.4103/0975-7406.137418. View

14.

Kaminagakura E, Almeida J, Carvalho Y, Franco R, Soares F, Rocha R . Keratocyst of the buccal mucosa: case report and immunohistochemical comparative study with sporadic intraosseous keratocystic odontogenic tumor. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013; 116(5):e387-92. DOI: 10.1016/j.oooo.2013.02.005. View

15.

Rao R, Patil S, Ganavi B . Oral cytokeratins in health and disease. J Contemp Dent Pract. 2014; 15(1):127-36. DOI: 10.5005/jp-journals-10024-1502. View

16.

da Silva M, de Sousa S, Correa L, Carvalhosa A, de Araujo V . Immunohistochemical study of the orthokeratinized odontogenic cyst: a comparison with the odontogenic keratocyst. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002; 94(6):732-7. DOI: 10.1067/moe.2002.125199. View

17.

Franke W, Schmid E, Osborn M, Weber K . Intermediate-sized filaments of human endothelial cells. J Cell Biol. 1979; 81(3):570-80. PMC: 2110384. DOI: 10.1083/jcb.81.3.570. View