Ultrastructural and Phenotypical Diversity of Macrophages in the Rat Ileal Mucosa

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 2021 May 7

PMID 33961127

Citations 5

Authors

Youhei Mantani

Tomohiro Haruta

Satoki Nakanishi

Nanami Sakata

Hideto Yuasa

Toshifumi Yokoyama

Nobuhiko Hoshi

Affiliations

Soon will be listed here.

Abstract

Several types of macrophages have been reported in the intestinal mucosa, but their histological localization remains ambiguous. Here, we obtained detailed information about ultrastructural and phenotypical diversity of macrophage-like cells (MLCs) in the rat ileal mucosa using immunofluorescent analysis and serial block-face scanning electron microscopy (SBF-SEM). The results revealed that the cells immunopositive for CD68, the pan-macrophage marker, included CD163CD4, CD163CD4, and CD163CD4 cells in the lamina propria (LP) of the intestinal villus and around the crypt. CD68CD4CD163 cells seemed to be preferentially localized in the intestinal villus, whereas CD68CD163CD4 cells were frequently localized around the crypt. SBF-SEM analysis identified three types of MLCs in the ileal mucosa, which were tentatively named types I-III MLC based on aspects of the 3D-ultrastructure, such as the localization, quantity of lysosomes, endoplasmic reticulum, and exoplasm. Type I and II MLCs were localized in the villous LP, while type III MLCs were localized around the crypt, although type II MLCs were a minor population. All three MLC types extended their cellular processes into the epithelium, with type I MLCs showing the greatest abundance of extended processes. Type I MLCs in the upper portion of the intestinal villus showed a higher level of attachment to intraepithelial lymphocytes (IELs) compared to type III MLCs around the crypt. These findings suggest that macrophages of the rat ileal mucosa differed by region along the longitudinal axis of the villous tip-crypt from the perspective of ultrastructure, cellular composition, localization, and interactions with IELs.

Citing Articles

Regional differences in the ultrastructure of mucosal macrophages in the rat large intestine.

Murase S, Mantani Y, Ohno N, Shimada A, Nakanishi S, Morishita R Cell Tissue Res. 2024; 396(2):245-253.

PMID: 38485763 DOI: 10.1007/s00441-024-03883-w.

Histological study on the reginal difference in the localization of mucosal enteric glial cells and their sheath structure in the rat intestine.

Mantani Y, Ohno N, Haruta T, Nakanishi S, Morishita R, Murase S J Vet Med Sci. 2023; 85(10):1034-1039.

PMID: 37612064 PMC: 10600526. DOI: 10.1292/jvms.23-0266.

Diurnal changes in bacterial settlement on the Peyer's patch and surrounding mucosa in the rat ileum and its effect against the intestinal immune system.

Mantani Y, Sakata N, Kubota N, Shimada A, Nakanishi S, Yokoyama T Cell Tissue Res. 2023; 393(1):83-95.

PMID: 37148397 DOI: 10.1007/s00441-023-03772-8.

Histological study on regional specificity of the mucosal nerve network in the rat large intestine.

Nakanishi S, Mantani Y, Ohno N, Morishita R, Yokoyama T, Hoshi N J Vet Med Sci. 2022; 85(2):123-134.

PMID: 36517005 PMC: 10017283. DOI: 10.1292/jvms.22-0433.

Region specificity of fibroblast-like cells in the mucosa of the rat large intestine.

Tamura S, Mantani Y, Nakanishi S, Ohno N, Yokoyama T, Hoshi N Cell Tissue Res. 2022; 389(3):427-441.

PMID: 35779135 DOI: 10.1007/s00441-022-03660-7.

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