Cryptic Biosynthesis of the Berkeleypenostatins from Coculture of Extremophilic Sp

Overview

Journal J Nat Prod

Date 2021 May 6

PMID 33957049

Citations 1

Authors

Andrea A Stierle

Donald B Stierle

Daniel Decato

Jeremy Alverson

Lily Apedaile

Affiliations

Soon will be listed here.

Abstract

Coculture fermentation of and yielded berkeleypenostatins A-G (-) as well as the previously reported berkeleylactones A-H, the known macrolide A26771B, citrinin, and patulin. As was true with the berkeleylactones, there was no evidence of the berkeleypenostatins in either axenic culture. The structures were deduced from analyses of spectral data, and the absolute configuration of berkeleypenostatin A () was determined by single-crystal X-ray crystallography. Berkeleypenostatins A () and E () inhibited migration of human pancreatic carcinoma cells (HPAF-II). Both compounds were tested by the NCI Developmental Therapeutics Program. In the NCI 60 cell five-dose screen, berkeleypenostatin E () was the more active of the two, with 1-10 μM total growth inhibition (TGI) of all leukemia cell lines, as well as the majority of colon, CNS, melanoma, ovarian, prostate, renal, and breast cancer cell lines.

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