Comparative Study of Gavage and Intraperitoneal Administration of Gamma-oryzanol in Alleviation/attenuation in a Rat Animal Model of Renal Ischemia/reperfusion-induced Injury

Overview

Journal Iran J Basic Med Sci

Publisher Mashhad University of Medical Sciences

Specialty General Medicine

Date 2021 May 6

PMID 33953856

Citations 18

Authors

Yasin Bagheri

Alireza Barati

Sana Nouraei

Nasim Jalili Namini

Mohammad Bakhshi

Ezzatollah Fathi

Soheila Montazersaheb

Affiliations

Soon will be listed here.

Abstract

Objectives: Ischemia/reperfusion (I/R) is the leading cause of acute kidney injury. This study aimed to elucidate the reno-protective effect of gamma-oryzanol (GO) by comparing gavage and intraperitoneal (IP) administration methods on renal I/R injury in a rat model.

Materials And Methods: Rats were divided into four groups including (group 1) sham, (group 2) I/R-control, (group 3) I/R+GO gavage-treated, and (group 4) I/R+ GO IP-treated. A single dose of GO was administrated to groups 3 and 4 (100 mg/kg body weight), 60 min before induction of I/R. After anesthesia, I/R was created by 45 min of ischemia, followed by 6 hr of reperfusion. Then, blood and tissue samples were subjected to evaluation of renal function, anti-oxidant capacity, inflammation, apoptotic proteins, and IKB/NF-kB pathway.

Results: The two GO administration methods showed improvement of renal function along with attenuation of histological abnormalities. An increase in antioxidant capacity along with a decrease in pro-inflammatory markers, decline in the expression levels of BAX, Bax/Bcl-2, and caspase-3, and up-regulation of Bcl-2 expression were recorded. Moreover, a significant decrease in NF-Kb, p-IKBα, and MMP-2/9 with an increase in IKBα levels were also observed. Overall, in a comparative evaluation between the two gavage and IP administration methods, we did not find any differences in all examined parameters, except IL-6 which had a better result via gavage.

Conclusion: A single dose of GO administration has a reno-protective effect against renal I/R injury. Gavage and IP administration exhibit similar efficiency in alleviation of I/R injury.

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References

Zolali E, Asgharian P, Hamishehkar H, Kouhsoltani M, Khodaii H, Hamishehkar H . Effects of gamma oryzanol on factors of oxidative stress and sepsis-induced lung injury in experimental animal model. Iran J Basic Med Sci. 2016; 18(12):1257-63. PMC: 4744368. View

Granger D, Kvietys P . Reperfusion injury and reactive oxygen species: The evolution of a concept. Redox Biol. 2015; 6:524-551. PMC: 4625011. DOI: 10.1016/j.redox.2015.08.020. View

Tarhriz V, Eyvazi S, Musavi M, Abasi M, Sharifi K, Ghanbarian H . Transient induction of Cdk9 in the early stage of differentiation is critical for myogenesis. J Cell Biochem. 2019; 120(11):18854-18861. DOI: 10.1002/jcb.29204. View

Gao D, Jing S, Zhang Q, Wu G . Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll-like receptor 4/nuclear factor-κB signaling pathway. Exp Ther Med. 2018; 15(1):1029-1035. PMC: 5780844. DOI: 10.3892/etm.2017.5479. View

Martin J, Gruszczyk A, Beach T, Murphy M, Saeb-Parsy K . Mitochondrial mechanisms and therapeutics in ischaemia reperfusion injury. Pediatr Nephrol. 2018; 34(7):1167-1174. PMC: 6366561. DOI: 10.1007/s00467-018-3984-5. View

Brazvan B, Farahzadi R, Mohammadi S, Montazer Saheb S, Shanehbandi D, Schmied L . Key Immune Cell Cytokines Affects the Telomere Activity of Cord Blood Cells In vitro. Adv Pharm Bull. 2016; 6(2):153-61. PMC: 4961972. DOI: 10.15171/apb.2016.022. View

Dennis J, Witting P . Protective Role for Antioxidants in Acute Kidney Disease. Nutrients. 2017; 9(7). PMC: 5537833. DOI: 10.3390/nu9070718. View

Lobo V, Patil A, Phatak A, Chandra N . Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev. 2012; 4(8):118-26. PMC: 3249911. DOI: 10.4103/0973-7847.70902. View

Farahzadi R, Fathi E, Vietor I . Mesenchymal Stem Cells Could Be Considered as a Candidate for Further Studies in Cell-Based Therapy of Alzheimer's Disease via Targeting the Signaling Pathways. ACS Chem Neurosci. 2020; 11(10):1424-1435. DOI: 10.1021/acschemneuro.0c00052. View

10.

Fathi E, Valipour B, Sanaat Z, Nozad Charoudeh H, Farahzadi R . Interleukin-6, -8, and TGF-β Secreted from Mesenchymal Stem Cells Show Functional Role in Reduction of Telomerase Activity of Leukemia Cell Via Wnt5a/β-Catenin and P53 Pathways. Adv Pharm Bull. 2020; 10(2):307-314. PMC: 7191235. DOI: 10.34172/apb.2020.037. View

11.

Van Opdenbosch N, Lamkanfi M . Caspases in Cell Death, Inflammation, and Disease. Immunity. 2019; 50(6):1352-1364. PMC: 6611727. DOI: 10.1016/j.immuni.2019.05.020. View

12.

Yaribeygi H, Farrokhi F, Rezaee R, Sahebkar A . Oxidative stress induces renal failure: A review of possible molecular pathways. J Cell Biochem. 2017; 119(4):2990-2998. DOI: 10.1002/jcb.26450. View

13.

Kinra M, Arora D, Mudgal J, Pai K, Rao C, Nampoothiri M . Effect of Caffeic Acid on Ischemia-Reperfusion-Induced Acute Renal Failure in Rats. Pharmacology. 2019; 103(5-6):315-319. DOI: 10.1159/000497474. View

14.

Islam M, Murata T, Fujisawa M, Nagasaka R, Ushio H, Bari A . Anti-inflammatory effects of phytosteryl ferulates in colitis induced by dextran sulphate sodium in mice. Br J Pharmacol. 2008; 154(4):812-24. PMC: 2439859. DOI: 10.1038/bjp.2008.137. View

15.

Erol N, Saglam L, Saglam Y, Erol H, Altun S, Aktas M . The Protection Potential of Antioxidant Vitamins Against Acute Respiratory Distress Syndrome: a Rat Trial. Inflammation. 2019; 42(5):1585-1594. DOI: 10.1007/s10753-019-01020-2. View

16.

Francisqueti F, Ferron A, Hasimoto F, Alves P, Garcia J, Dos Santos K . Gamma Oryzanol Treats Obesity-Induced Kidney Injuries by Modulating the Adiponectin Receptor 2/PPAR- Axis. Oxid Med Cell Longev. 2018; 2018:1278392. PMC: 6151244. DOI: 10.1155/2018/1278392. View

17.

Malek M, Nematbakhsh M . Renal ischemia/reperfusion injury; from pathophysiology to treatment. J Renal Inj Prev. 2015; 4(2):20-7. PMC: 4459724. DOI: 10.12861/jrip.2015.06. View

18.

Kaya C, Karabulut R, Turkyilmaz Z, Sonmez K, Kulduk G, Gulbahar O . Lycopene has reduced renal damage histopathologically and biochemically in experimental renal ischemia-reperfusion injury. Ren Fail. 2015; 37(8):1390-5. DOI: 10.3109/0886022X.2015.1064742. View

19.

Wu M, Yiang G, Liao W, Tsai A, Cheng Y, Cheng P . Current Mechanistic Concepts in Ischemia and Reperfusion Injury. Cell Physiol Biochem. 2018; 46(4):1650-1667. DOI: 10.1159/000489241. View

20.

Havasi A, Borkan S . Apoptosis and acute kidney injury. Kidney Int. 2011; 80(1):29-40. PMC: 4625984. DOI: 10.1038/ki.2011.120. View