DNA Identification of Species of the Anopheles Maculipennis Complex and First Record of An. Daciae in Belgium

Overview

Journal Med Vet Entomol

Specialties Biology
Veterinary Medicine

Date 2021 May 5

PMID 33951205

Citations 5

Authors

N Smitz

K De Wolf

A Gheysen

I Deblauwe

A Vanslembrouck

K Meganck

J De Witte

A Schneider

I Verle

W Dekoninck

S Gombeer

A Vanderheyden

M De Meyer

T Backeljau

R Muller

W Van Bortel

Affiliations

Soon will be listed here.

Abstract

The present study aimed at identifying the members of the Anopheles maculipennis complex (Diptera: Culicidae) occurring in Belgium. Therefore, the second internal transcribed spacer of nuclear ribosomal DNA (ITS2) and the mitochondrial cytochrome oxidase subunit I (COI) loci were sequenced in 175 and 111 specimens, respectively, collected between 2007 and 2019. In parallel, the suitability of two species-diagnostic PCR-RFLP assays was tested. The identified specimens included: An. maculipennis s.s. (N = 105), An. daciae (N = 62), An. atroparvus (N = 6) and An. messeae (N = 2). Each species was characterized by unique ITS2 haplotypes, whereas COI only supported the monophyly of An. atroparvus, a historical malaria vector in Belgium. Species identification results were further supported by unique PCR-RFLP banding patterns. We report for the first time An. daciae in Belgium, where it was found to co-occur with An. maculipennis s.s. The latter was the most prevalent in the collection studied (60%) and appears to have the widest distribution in Belgium. As in other studies, An. daciae and An. messeae appeared the most closely related species, up to the point that their species status remains debatable, while their ecological differences, including vector competences, need further study.

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References

Nicolescu G, Linton Y, Vladimirescu A, Howard T, Harbach R . Mosquitoes of the Anopheles maculipennis group (Diptera: Culicidae) in Romania, with the discovery and formal recognition of a new species based on molecular and morphological evidence. Bull Entomol Res. 2004; 94(6):525-35. DOI: 10.1079/ber2004330. View

Chaves L, Harrington L, Keogh C, Nguyen A, Kitron U . Blood feeding patterns of mosquitoes: random or structured?. Front Zool. 2010; 7:3. PMC: 2826349. DOI: 10.1186/1742-9994-7-3. View

Versteirt V, Boyer S, Damiens D, De Clercq E, Dekoninck W, Ducheyne E . Nationwide inventory of mosquito biodiversity (Diptera: Culicidae) in Belgium, Europe. Bull Entomol Res. 2012; 103(2):193-203. PMC: 3580911. DOI: 10.1017/S0007485312000521. View

Takken W, Verhulst N . Host preferences of blood-feeding mosquitoes. Annu Rev Entomol. 2012; 58:433-53. DOI: 10.1146/annurev-ento-120811-153618. View

Lindsay S, Birley M . Climate change and malaria transmission. Ann Trop Med Parasitol. 1996; 90(6):573-88. DOI: 10.1080/00034983.1996.11813087. View

Takken W, Geene R, Adam W, Jetten T, van der Velden J . Distribution and dynamics of larval populations of Anopheles messeae and A. atroparvus in the delta of the rivers Rhine and Meuse, The Netherlands. Ambio. 2002; 31(3):212-8. DOI: 10.1579/0044-7447-31.3.212. View

Jost H, Bialonski A, Storch V, Gunther S, Becker N, Schmidt-Chanasit J . Isolation and phylogenetic analysis of Sindbis viruses from mosquitoes in Germany. J Clin Microbiol. 2010; 48(5):1900-3. PMC: 2863933. DOI: 10.1128/JCM.00037-10. View

Lilja T, Eklof D, Jaenson T, Lindstrom A, Terenius O . Single nucleotide polymorphism analysis of the ITS2 region of two sympatric malaria mosquito species in Sweden: Anopheles daciae and Anopheles messeae. Med Vet Entomol. 2020; 34(3):364-368. DOI: 10.1111/mve.12436. View

Ibanez-Justicia A, Smitz N, den Hartog W, van de Vossenberg B, De Wolf K, Deblauwe I . Detection of Exotic Mosquito Species (Diptera: Culicidae) at International Airports in Europe. Int J Environ Res Public Health. 2020; 17(10). PMC: 7277938. DOI: 10.3390/ijerph17103450. View

10.

Brugman V, Hernandez-Triana L, Prosser S, Weland C, Westcott D, Fooks A . Molecular species identification, host preference and detection of myxoma virus in the Anopheles maculipennis complex (Diptera: Culicidae) in southern England, UK. Parasit Vectors. 2015; 8:421. PMC: 4536751. DOI: 10.1186/s13071-015-1034-8. View

11.

Deblauwe I, Demeulemeester J, De Witte J, Hendy A, Sohier C, Madder M . Increased detection of Aedes albopictus in Belgium: no overwintering yet, but an intervention strategy is still lacking. Parasitol Res. 2015; 114(9):3469-77. DOI: 10.1007/s00436-015-4575-z. View

12.

Collins F, Paskewitz S . A review of the use of ribosomal DNA (rDNA) to differentiate among cryptic Anopheles species. Insect Mol Biol. 1996; 5(1):1-9. DOI: 10.1111/j.1365-2583.1996.tb00034.x. View

13.

Di Luca M, Boccolini D, Marinuccil M, Romi R . Intrapopulation polymorphism in Anopheles messeae (An. maculipennis complex) inferred by molecular analysis. J Med Entomol. 2004; 41(4):582-6. DOI: 10.1603/0022-2585-41.4.582. View

14.

Kumar S, Stecher G, Li M, Knyaz C, Tamura K . MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol Biol Evol. 2018; 35(6):1547-1549. PMC: 5967553. DOI: 10.1093/molbev/msy096. View

15.

Bezzhonova O, Goryacheva I . Intragenomic heterogeneity of rDNA internal transcribed spacer 2 in Anopheles messeae (Diptera: Culicidae). J Med Entomol. 2008; 45(3):337-41. DOI: 10.1603/0022-2585(2008)45[337:ihorit]2.0.co;2. View

16.

Filipe A . Isolation in Portugal of West Nile virus from Anopheles maculipennis mosquitoes. Acta Virol. 1972; 16(4):361. View

17.

Weitzel T, Gauch C, Becker N . Identification of Anopheles daciae in Germany through ITS2 sequencing. Parasitol Res. 2012; 111(6):2431-8. DOI: 10.1007/s00436-012-3102-8. View

18.

Kavran M, Zgomba M, Weitzel T, Petric D, Manz C, Becker N . Distribution of Anopheles daciae and other Anopheles maculipennis complex species in Serbia. Parasitol Res. 2018; 117(10):3277-3287. PMC: 6153502. DOI: 10.1007/s00436-018-6028-y. View

19.

Proft J, Maier W, Kampen H . Identification of six sibling species of the Anopheles maculipennis complex (Diptera: Culicidae) by a polymerase chain reaction assay. Parasitol Res. 1999; 85(10):837-43. DOI: 10.1007/s004360050642. View

20.

Luhken R, Czajka C, Steinke S, Jost H, Schmidt-Chanasit J, Pfitzner W . Distribution of individual members of the mosquito Anopheles maculipennis complex in Germany identified by newly developed real-time PCR assays. Med Vet Entomol. 2016; 30(2):144-54. DOI: 10.1111/mve.12161. View