Candidate Molecular Biomarkers for the Non- Invasive Detection of Colorectal Cancer Using Gene Expression Profiling

Overview

Journal Iran J Pathol

Date 2021 May 3

PMID 33936232

Citations 1

Authors

Mohammad Shafiei

Mahdi Alemrajabi

Ali Najafi

Amir Homayoun Keihan

Masoud Reza Sohrabi

Affiliations

Soon will be listed here.

Abstract

Background & Objective: olorectal Cancer (CRC) is the third most common cancer after prostate (breast in women) and lung cancer; it is also the third cause of cancer deaths reported in both men and women in 2020. Currently, the most commonly used diagnostic tools for CRC are colonoscopy, serological methods, and other imaging techniques. Despite the benefits and abilities of these methods, each of them has disadvantages that reduce its functionality and acceptance. The aim of this study was identifying specific and non-invasive genetic biomarkers to diagnose colorectal cancer.

Methods: In this study, changes in the expression of and genes were evaluated by Real Time PCR in blood and tissue samples of CRC patients. A total of 100 samples (50 Blood and 50 Tissue samples) were evaluated with a definite diagnosis of CRC in Firoozgar Hspital, Tehran, Iran, in 2018. The QPCR method was used to compare the expression of candidate genes between the patients group and control group in both samples. Sensitivity and specificity of the test were examined using ROC curve analysis.

Results: The results showed a significant down-regulation in the expression of both selected genes in tissue and peripheral blood in the various stages of the CRC. The sensitivity and specifity of both genes was about 80%.

Conclusion: The findings showed that the two candidate genes can be suggested as specific biomarkers for diagnosis of CRC using the peripheral blood as a non-invasive method. For a definite conclusion, more research is needed.

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References

Rasmussen S, Krarup H, Sunesen K, Johansen M, Stender M, Pedersen I . The prognostic efficacy of cell-free DNA hypermethylation in colorectal cancer. Oncotarget. 2018; 9(6):7010-7022. PMC: 5805532. DOI: 10.18632/oncotarget.24097. View

Kahi C, Imperiale T, Juliar B, Rex D . Effect of screening colonoscopy on colorectal cancer incidence and mortality. Clin Gastroenterol Hepatol. 2009; 7(7):770-5. DOI: 10.1016/j.cgh.2008.12.030. View

Song L, Yu H, Jia J, Li Y . A systematic review of the performance of the SEPT9 gene methylation assay in colorectal cancer screening, monitoring, diagnosis and prognosis. Cancer Biomark. 2017; 18(4):425-432. DOI: 10.3233/CBM-160321. View

Wu D, Zhou G, Jin P, Zhu J, Li S, Wu Q . Detection of Colorectal Cancer Using a Simplified SEPT9 Gene Methylation Assay Is a Reliable Method for Opportunistic Screening. J Mol Diagn. 2016; 18(4):535-45. DOI: 10.1016/j.jmoldx.2016.02.005. View

Rasmussen S, Krarup H, Sunesen K, Pedersen I, Madsen P, Thorlacius-Ussing O . Hypermethylated DNA as a biomarker for colorectal cancer: a systematic review. Colorectal Dis. 2016; 18(6):549-61. DOI: 10.1111/codi.13336. View

Okugawa Y, Grady W, Goel A . Epigenetic Alterations in Colorectal Cancer: Emerging Biomarkers. Gastroenterology. 2015; 149(5):1204-1225.e12. PMC: 4589488. DOI: 10.1053/j.gastro.2015.07.011. View

Leung W, To K, Man E, Chan M, Bai A, Hui A . Quantitative detection of promoter hypermethylation in multiple genes in the serum of patients with colorectal cancer. Am J Gastroenterol. 2005; 100(10):2274-9. DOI: 10.1111/j.1572-0241.2005.50412.x. View

Duffy M . Carcinoembryonic antigen as a marker for colorectal cancer: is it clinically useful?. Clin Chem. 2001; 47(4):624-30. View

Han M, Liew C, Zhang H, Chao S, Zheng R, Yip K . Novel blood-based, five-gene biomarker set for the detection of colorectal cancer. Clin Cancer Res. 2008; 14(2):455-60. DOI: 10.1158/1078-0432.CCR-07-1801. View

10.

Itzkowitz S, Jandorf L, Brand R, Rabeneck L, Schroy 3rd P, Sontag S . Improved fecal DNA test for colorectal cancer screening. Clin Gastroenterol Hepatol. 2006; 5(1):111-7. DOI: 10.1016/j.cgh.2006.10.006. View

11.

Ahmed D, Danielsen S, Aagesen T, Bretthauer M, Thiis-Evensen E, Hoff G . A tissue-based comparative effectiveness analysis of biomarkers for early detection of colorectal tumors. Clin Transl Gastroenterol. 2013; 3:e27. PMC: 3535074. DOI: 10.1038/ctg.2012.21. View

12.

Senore C, Ederle A, Fantin A, Andreoni B, Bisanti L, Grazzini G . Acceptability and side-effects of colonoscopy and sigmoidoscopy in a screening setting. J Med Screen. 2011; 18(3):128-34. DOI: 10.1258/jms.2011.010135. View

13.

Siegel R, Miller K, Fedewa S, Ahnen D, Meester R, Barzi A . Colorectal cancer statistics, 2017. CA Cancer J Clin. 2017; 67(3):177-193. DOI: 10.3322/caac.21395. View

14.

Lofton-Day C, Model F, deVos T, Tetzner R, Distler J, Schuster M . DNA methylation biomarkers for blood-based colorectal cancer screening. Clin Chem. 2007; 54(2):414-23. DOI: 10.1373/clinchem.2007.095992. View

15.

Sartore-Bianchi A, Marsoni S, Siena S . Human Epidermal Growth Factor Receptor 2 as a Molecular Biomarker for Metastatic Colorectal Cancer. JAMA Oncol. 2017; 4(1):19-20. DOI: 10.1001/jamaoncol.2017.3323. View

16.

Sandhu S, Wu X, Nabi Z, Rastegar M, Kung S, Mai S . Loss of HLTF function promotes intestinal carcinogenesis. Mol Cancer. 2012; 11:18. PMC: 3337324. DOI: 10.1186/1476-4598-11-18. View

17.

Herbst A, Kolligs F . Detection of DNA hypermethylation in remote media of patients with colorectal cancer: new biomarkers for colorectal carcinoma. Tumour Biol. 2012; 33(2):297-305. DOI: 10.1007/s13277-012-0346-y. View

18.

Philipp A, Stieber P, Nagel D, Neumann J, Spelsberg F, Jung A . Prognostic role of methylated free circulating DNA in colorectal cancer. Int J Cancer. 2012; 131(10):2308-19. DOI: 10.1002/ijc.27505. View

19.

Danese E, Montagnana M . Epigenetics of colorectal cancer: emerging circulating diagnostic and prognostic biomarkers. Ann Transl Med. 2017; 5(13):279. PMC: 5515803. DOI: 10.21037/atm.2017.04.45. View

20.

Hall P, Jung K, Hillan K, Russell S . Expression profiling the human septin gene family. J Pathol. 2005; 206(3):269-78. DOI: 10.1002/path.1789. View