Systematic Analysis of Migration Factors by MigExpress Identifies Essential Cell Migration Control Genes in Non-small Cell Lung Cancer

Overview

Journal Mol Oncol

Specialties Molecular Biology
Oncology

Date 2021 May 2

PMID 33934493

Citations 7

Authors

Jagriti Pal

Andrea C Becker

Sonam Dhamija

Jeanette Seiler

Mahmoud Abdelkarim

Yogita Sharma

Jurgen Behr

Chen Meng

Christina Ludwig

Bernhard Kuster

Sven Diederichs

Affiliations

Soon will be listed here.

Abstract

Cell migration is an essential process in health and in disease, including cancer metastasis. A comprehensive inventory of migration factors is nonetheless lacking-in part due to the difficulty in assessing migration using high-throughput technologies. Hence, there are currently very few screens that systematically reveal factors controlling cell migration. Here, we introduce MigExpress as a platform for the 'identification of Migration control genes by differential Expression'. MigExpress exploits the combination of in-depth molecular profiling and the robust quantitative analysis of migration capacity in a broad panel of samples and identifies migration-associated genes by their differential expression in slow- versus fast-migrating cells. We applied MigExpress to investigate non-small cell lung cancer (NSCLC), which is the most frequent cause of cancer mortality mainly due to metastasis. In 54 NSCLC cell lines, we comprehensively determined mRNA and protein expression. Correlating the transcriptome and proteome profiles with the quantified migration properties led to the discovery and validation of FLNC, DSE, CPA4, TUBB6, and BICC1 as migration control factors in NSCLC cells, which were also negatively correlated with patient survival. Notably, FLNC was the least expressed filamin in NSCLC, but the only one controlling cell migration and correlating with patient survival and metastatic disease stage. In our study, we present MigExpress as a new method for the systematic analysis of migration factors and provide a comprehensive resource of transcriptomic and proteomic data of NSCLC cell lines related to cell migration.

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