Electroporation-mediated Transfection of Acholeplasma Laidlawii with Mycoplasma Virus L1 and L3 DNA

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1988 Aug 1

PMID 3392773

Citations 4

Authors

A Lorenz

W Just

M da Silva Cardoso

G KLOTZ

Affiliations

Soon will be listed here.

Abstract

In contrast to mycoplasma virus L1 and L2 circular DNA, mycoplasma virus L3 linear DNA is not biologically active in polyethylene glycol-mediated transfection. Electroporation of Acholeplasma laidlawii, however, leads to plaque formation after incubation with L3 DNA. The efficiency of electroporation-mediated transfection is 1/10 that of polyethylene glycol-mediated transfection as estimated with L1 DNA. Trypsin treatment of cells before DNA addition increases the efficiency of DNA uptake.

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References

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