A Study on the Synbiotic Composition of and Fructans from Roots and Tubers Against

Overview

Journal Microorganisms

Specialty Microbiology

Date 2021 Apr 30

PMID 33926121

Citations 3

Authors

Svetlana A Evdokimova

Vera S Nokhaeva

Boris A Karetkin

Elena V Guseva

Natalia V Khabibulina

Maria A Kornienko

Veronika D Grosheva

Natalia V Menshutina

Irina V Shakir

Victor I Panfilov

Affiliations

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Abstract

A number of mechanisms have been proposed explaining probiotics and prebiotics benefit human health, in particular, probiotics have a suppression effect on pathogen growth that can be enhanced with the introduction of prebiotics. In vitro models enhanced with computational biology can be useful for selecting a composition with prebiotics from new plant sources with the greatest synergism. Water extracts from burdock root and Jerusalem artichoke tubers were purified by ultrafiltration and activated charcoal and concentrated on a rotary evaporator. Fructans were precipitated with various concentrations of ethanol. 8 VKPM AC-2136 and ATCC 43300 strains were applied to estimate the synbiotic effect. The growth of bifidobacteria and staphylococci in monocultures and cocultures in broths with glucose, commercial prebiotics, as well as isolated fructans were studied. The minimum inhibitory concentrations (MICs) of lactic and acetic acids for the strain were determined. A quantitative model joining the formation of organic acids by probiotics as antagonism factors and the MICs of pathogens (as the measure of their inhibition) was tested in cocultures and showed a high predictive value (R ≥ 0.86). The synbiotic factor obtained from the model was calculated based on the experimental data and obtained constants. Fructans precipitated with 20% ethanol and have the greater synergism against .

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