A Carbon-Based Antifouling Nano-Biosensing Interface for Label-Free POCT of HbA1c

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2021 Apr 30

PMID 33921226

Citations 6

Authors

Zhenhua Li

Jianyong Li

Yanzhi Dou

Lihua Wang

Shiping Song

Affiliations

Soon will be listed here.

Abstract

Electrochemical biosensing relies on electron transport on electrode surfaces. However, electrode inactivation and biofouling caused by a complex biological sample severely decrease the efficiency of electron transfer and the specificity of biosensing. Here, we designed a three-dimensional antifouling nano-biosensing interface to improve the efficiency of electron transfer by a layer of bovine serum albumin (BSA) and multi-walled carbon nanotubes (MWCNTs) cross-linked with glutaraldehyde (GA). The electrochemical properties of the BSA/MWCNTs/GA layer were investigated using both cyclic voltammetry and electrochemical impedance to demonstrate its high-efficiency antifouling nano-biosensing interface. The BSA/MWCNTs/GA layer kept 92% of the original signal in 1% BSA and 88% of that in unprocessed human serum after a 1-month exposure, respectively. Importantly, we functionalized the BSA/MWCNTs/GA layer with HbA1c antibody (anti-HbA1c) and 3-aminophenylboronic acid (APBA) for sensitive detection of glycated hemoglobin A (HbA1c). The label-free direct electrocatalytic oxidation of HbA1c was investigated by cyclic voltammetry (CV). The linear dynamic range of 2 to 15% of blood glycated hemoglobin A (HbA1c) in non-glycated hemoglobin (HbAo) was determined. The detection limit was 0.4%. This high degree of differentiation would facilitate a label-free POCT detection of HbA1c.

Citing Articles

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References

Salvati A, Pitek A, Monopoli M, Prapainop K, Bombelli F, Hristov D . Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface. Nat Nanotechnol. 2013; 8(2):137-43. DOI: 10.1038/nnano.2012.237. View

Su J, Liu W, Chen S, Deng W, Dou Y, Zhao Z . A Carbon-Based DNA Framework Nano-Bio Interface for Biosensing with High Sensitivity and a High Signal-to-Noise Ratio. ACS Sens. 2020; 5(12):3979-3987. DOI: 10.1021/acssensors.0c01745. View

Kumar T, K Sundramoorthy A . Electrochemical biosensor for methyl parathion based on single-walled carbon nanotube/glutaraldehyde crosslinked acetylcholinesterase-wrapped bovine serum albumin nanocomposites. Anal Chim Acta. 2019; 1074:131-141. DOI: 10.1016/j.aca.2019.05.011. View

Moon J, Kim D, Kim M, Han J, Jung D, Shim Y . A disposable amperometric dual-sensor for the detection of hemoglobin and glycated hemoglobin in a finger prick blood sample. Biosens Bioelectron. 2016; 91:128-135. DOI: 10.1016/j.bios.2016.12.038. View

Yang F, Zuo X, Li Z, Deng W, Shi J, Zhang G . A bubble-mediated intelligent microscale electrochemical device for single-step quantitative bioassays. Adv Mater. 2014; 26(27):4671-6. DOI: 10.1002/adma.201400451. View

Mout R, Moyano D, Rana S, Rotello V . Surface functionalization of nanoparticles for nanomedicine. Chem Soc Rev. 2012; 41(7):2539-44. PMC: 4102397. DOI: 10.1039/c2cs15294k. View

Li J, Wang J, Guo X, Zheng Q, Peng J, Tang H . Carbon Nanotubes Labeled with Aptamer and Horseradish Peroxidase as a Probe for Highly Sensitive Protein Biosensing by Postelectropolymerization of Insoluble Precipitates on Electrodes. Anal Chem. 2015; 87(15):7610-7. DOI: 10.1021/acs.analchem.5b00640. View

Hwang H, Jeong J, Kim Y, Chang M . Carbon Nanomaterials as Versatile Platforms for Biosensing Applications. Micromachines (Basel). 2020; 11(9). PMC: 7569884. DOI: 10.3390/mi11090814. View

Kaur J, Jiang C, Liu G . Different strategies for detection of HbA1c emphasizing on biosensors and point-of-care analyzers. Biosens Bioelectron. 2018; 123:85-100. DOI: 10.1016/j.bios.2018.06.018. View

10.

Furst A, Landefeld S, Hill M, Barton J . Electrochemical patterning and detection of DNA arrays on a two-electrode platform. J Am Chem Soc. 2013; 135(51):19099-102. PMC: 4026266. DOI: 10.1021/ja410902j. View

11.

Ziegler J, Andoni I, Choi E, Fang L, Flores-Zuleta H, Humphrey N . Sensors Based Upon Nanowires, Nanotubes, and Nanoribbons: 2016-2020. Anal Chem. 2020; 93(1):124-166. DOI: 10.1021/acs.analchem.0c04476. View

12.

Tenzer S, Docter D, Kuharev J, Musyanovych A, Fetz V, Hecht R . Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology. Nat Nanotechnol. 2013; 8(10):772-81. DOI: 10.1038/nnano.2013.181. View

13.

Hu Y, Xu X, Liu Q, Wang L, Lin Z, Chen G . Ultrasensitive electrochemical biosensor for detection of DNA from Bacillus subtilis by coupling target-induced strand displacement and nicking endonuclease signal amplification. Anal Chem. 2014; 86(17):8785-90. DOI: 10.1021/ac502008k. View

14.

Mahshid S, Ricci F, Kelley S, Vallee-Belisle A . Electrochemical DNA-Based Immunoassay That Employs Steric Hindrance To Detect Small Molecules Directly in Whole Blood. ACS Sens. 2017; 2(6):718-723. DOI: 10.1021/acssensors.7b00176. View

15.

Wan Y, Wang H, Ji J, Kang K, Yang M, Huang Y . Zippering DNA Tetrahedral Hyperlink for Ultrasensitive Electrochemical MicroRNA Detection. Anal Chem. 2020; 92(22):15137-15144. DOI: 10.1021/acs.analchem.0c03553. View

16.

Liu G, Wan Y, Gau V, Zhang J, Wang L, Song S . An enzyme-based E-DNA sensor for sequence-specific detection of femtomolar DNA targets. J Am Chem Soc. 2008; 130(21):6820-5. DOI: 10.1021/ja800554t. View

17.

Zhang X, Xie M, Yang Z, Wu H, Fang C, Bai L . Antifouling Double-Skinned Forward Osmosis Membranes by Constructing Zwitterionic Brush-Decorated MWCNT Ultrathin Films. ACS Appl Mater Interfaces. 2019; 11(21):19462-19471. DOI: 10.1021/acsami.9b03259. View

18.

Mahshid S, Camire S, Ricci F, Vallee-Belisle A . A Highly Selective Electrochemical DNA-Based Sensor That Employs Steric Hindrance Effects to Detect Proteins Directly in Whole Blood. J Am Chem Soc. 2015; 137(50):15596-9. DOI: 10.1021/jacs.5b04942. View

19.

Tavallaie R, McCarroll J, Le Grand M, Ariotti N, Schuhmann W, Bakker E . Nucleic acid hybridization on an electrically reconfigurable network of gold-coated magnetic nanoparticles enables microRNA detection in blood. Nat Nanotechnol. 2018; 13(11):1066-1071. DOI: 10.1038/s41565-018-0232-x. View

20.

Deng W, Xu B, Hu H, Li J, Hu W, Song S . Diagnosis of schistosomiasis japonica with interfacial co-assembly-based multi-channel electrochemical immunosensor arrays. Sci Rep. 2013; 3:1789. PMC: 3646279. DOI: 10.1038/srep01789. View