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Mass Spectrometry Imaging As a Tool to Investigate Region Specific Lipid Alterations in Symptomatic Human Carotid Atherosclerotic Plaques

Overview
Journal Metabolites
Publisher MDPI
Date 2021 Apr 30
PMID 33919525
Citations 10
Authors
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Abstract

Atherosclerosis is characterized by fatty plaques in large and medium sized arteries. Their rupture can causes thrombi, occlusions of downstream vessels and adverse clinical events. The investigation of atherosclerotic plaques is made difficult by their highly heterogeneous nature. Here we propose a spatially resolved approach based on matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging to investigate lipids in specific regions of atherosclerotic plaques. The method was applied to a small dataset including symptomatic and asymptomatic human carotid atherosclerosis plaques. Tissue sections of symptomatic and asymptomatic human carotid atherosclerotic plaques were analyzed by MALDI mass spectrometry imaging (MALDI MSI) of lipids, and adjacent sections analyzed by histology and immunofluorescence. These multimodal datasets were used to compare the lipid profiles of specific histopathological regions within the plaque. The lipid profiles of macrophage-rich regions and intimal vascular smooth muscle cells exhibited the largest changes associated with plaque outcome. Macrophage-rich regions from symptomatic lesions were found to be enriched in sphingomyelins, and intimal vascular smooth muscle cells of symptomatic plaques were enriched in cholesterol and cholesteryl esters. The proposed method enabled the MALDI MSI analysis of specific regions of the atherosclerotic lesion, confirming MALDI MSI as a promising tool for the investigation of histologically heterogeneous atherosclerotic plaques.

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References
1.
Chistiakov D, Orekhov A, Bobryshev Y . Vascular smooth muscle cell in atherosclerosis. Acta Physiol (Oxf). 2015; 214(1):33-50. DOI: 10.1111/apha.12466. View

2.
Liebisch G, Vizcaino J, Kofeler H, Trotzmuller M, Griffiths W, Schmitz G . Shorthand notation for lipid structures derived from mass spectrometry. J Lipid Res. 2013; 54(6):1523-1530. PMC: 3646453. DOI: 10.1194/jlr.M033506. View

3.
Visscher M, Moerman A, Burgers P, van Beusekom H, Luider T, Verhagen H . Data Processing Pipeline for Lipid Profiling of Carotid Atherosclerotic Plaque with Mass Spectrometry Imaging. J Am Soc Mass Spectrom. 2019; 30(9):1790-1800. PMC: 6695360. DOI: 10.1007/s13361-019-02254-y. View

4.
Martin-Lorenzo M, Alvarez-Llamas G, McDonnell L, Vivanco F . Molecular histology of arteries: mass spectrometry imaging as a novel ex vivo tool to investigate atherosclerosis. Expert Rev Proteomics. 2015; 13(1):69-81. DOI: 10.1586/14789450.2016.1116944. View

5.
McMillen J, Fincher J, Klein D, Spraggins J, Caprioli R . Effect of MALDI matrices on lipid analyses of biological tissues using MALDI-2 postionization mass spectrometry. J Mass Spectrom. 2020; 55(12):e4663. PMC: 8099046. DOI: 10.1002/jms.4663. View