Existence of Multiple ESBL Genes Among Phenotypically Confirmed ESBL Producing and Concurrently Isolated from Clinical, Colonization and Contamination Samples from Neonatal Units at Bugando Medical Center, Mwanza, Tanzania

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2021 Apr 30

PMID 33919117

Citations 21

Authors

Vitus Silago

Dory Kovacs

Happyness Samson

Jeremiah Seni

Louise Matthews

Katarina Oravcova

Athumani M Lupindu

Abubakar S Hoza

Stephen E Mshana

Affiliations

Soon will be listed here.

Abstract

The proportions and similarities of extended-spectrum β-lactamase (ESBL) producing (ESBL-KP) and (ESBL-EC) carrying multiple ESBL genes is poorly known at our setting. This study investigated the existence of multiple ESBL genes (, and ) among ESBL-KP and ESBL-EC concurrently isolated from clinical, colonization, and contamination samples from neonatology units in Mwanza-Tanzania. Twenty and 55 presumptive ESBL-EC and ESBL-KP, respectively, from a previous study archived at -80 °C were successfully recovered for this study. Isolates were screened and confirmed for production of ESBLs by phenotypic methods followed by multiplex PCR assay to determine ESBL genes. All (100%) and 97.3% of presumptive ESBL isolates were phenotypically confirmed by Clinical and Laboratory Standards Institute (CLSI) and modified double-disc synergy methods, respectively. About 93.3% (70/75) of phenotypically confirmed ESBL isolates had at least one ESBL gene, whereby for 62.9% (44/70), all ESBL genes (, and ) were detected. Eight pairs of ESBL bacteria show similar patterns of antibiotics susceptibility and ESBL genes. ESBL-KP and ESBL-EC, concurrently isolated from clinical, colonization and contamination samples, harbored multiple ESBL genes. Further, eight pairs of ESBL isolates had similar patterns of antibiotics susceptibility and ESBL genes, suggesting transmission of and/or sharing of mobile genetic elements (MGEs) among ESBL-KP and ESBL-EC.

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References

Rawat D, Nair D . Extended-spectrum β-lactamases in Gram Negative Bacteria. J Glob Infect Dis. 2010; 2(3):263-74. PMC: 2946684. DOI: 10.4103/0974-777X.68531. View

Moremi N, Manda E, Falgenhauer L, Ghosh H, Imirzalioglu C, Matee M . Predominance of CTX-M-15 among ESBL Producers from Environment and Fish Gut from the Shores of Lake Victoria in Mwanza, Tanzania. Front Microbiol. 2016; 7:1862. PMC: 5130978. DOI: 10.3389/fmicb.2016.01862. View

Park H, Kim J, Ryu S, Jeon B . Predominance of bla and bla in extended-spectrum β-lactamase-producing Escherichia coli from raw retail chicken in South Korea. J Glob Antimicrob Resist. 2019; 17:216-220. DOI: 10.1016/j.jgar.2019.01.005. View

Monstein H, Ostholm-Balkhed A, Nilsson M, Nilsson M, Dornbusch K, Nilsson L . Multiplex PCR amplification assay for the detection of blaSHV, blaTEM and blaCTX-M genes in Enterobacteriaceae. APMIS. 2008; 115(12):1400-8. DOI: 10.1111/j.1600-0463.2007.00722.x. View

Zhuo C, Li X, Zong Z, Zhong N . Epidemic plasmid carrying bla(CTX-M-15) in Klebsiella penumoniae in China. PLoS One. 2013; 8(1):e52222. PMC: 3558504. DOI: 10.1371/journal.pone.0052222. View

Polsfuss S, Bloemberg G, Giger J, Meyer V, Bottger E, Hombach M . Practical approach for reliable detection of AmpC beta-lactamase-producing Enterobacteriaceae. J Clin Microbiol. 2011; 49(8):2798-803. PMC: 3147735. DOI: 10.1128/JCM.00404-11. View

Ghasemi Y, Archin T, Kargar M, Mohkam M . A simple multiplex PCR for assessing prevalence of extended-spectrum β-lactamases producing Klebsiella pneumoniae in Intensive Care Units of a referral hospital in Shiraz, Iran. Asian Pac J Trop Med. 2013; 6(9):703-8. DOI: 10.1016/S1995-7645(13)60122-4. View

Krishnamurthy V, G S V, Kumar M S, H V P, R P, E R N . Phenotypic and Genotypic Methods for Detection of Extended Spectrum β Lactamase Producing Escherichia coli and Klebsiella pneumoniae Isolated from Ventilator Associated Pneumonia. J Clin Diagn Res. 2013; 7(9):1975-8. PMC: 3809652. DOI: 10.7860/JCDR/2013/6544.3376. View

Gomez-Zorrilla S, Camoez M, Tubau F, Canizares R, Periche E, Dominguez M . Prospective observational study of prior rectal colonization status as a predictor for subsequent development of Pseudomonas aeruginosa clinical infections. Antimicrob Agents Chemother. 2015; 59(9):5213-9. PMC: 4538513. DOI: 10.1128/AAC.04636-14. View

10.

Moremi N, Claus H, Vogel U, Mshana S . Faecal carriage of CTX-M extended-spectrum beta-lactamase-producing Enterobacteriaceae among street children dwelling in Mwanza city, Tanzania. PLoS One. 2017; 12(9):e0184592. PMC: 5595323. DOI: 10.1371/journal.pone.0184592. View

11.

Kayange N, Kamugisha E, Mwizamholya D, Jeremiah S, Mshana S . Predictors of positive blood culture and deaths among neonates with suspected neonatal sepsis in a tertiary hospital, Mwanza-Tanzania. BMC Pediatr. 2010; 10:39. PMC: 2889942. DOI: 10.1186/1471-2431-10-39. View

12.

Bhalla A, Pultz N, Gries D, Ray A, Eckstein E, Aron D . Acquisition of nosocomial pathogens on hands after contact with environmental surfaces near hospitalized patients. Infect Control Hosp Epidemiol. 2004; 25(2):164-7. DOI: 10.1086/502369. View

13.

Seni J, Falgenhauer L, Simeo N, Mirambo M, Imirzalioglu C, Matee M . Multiple ESBL-Producing Escherichia coli Sequence Types Carrying Quinolone and Aminoglycoside Resistance Genes Circulating in Companion and Domestic Farm Animals in Mwanza, Tanzania, Harbor Commonly Occurring Plasmids. Front Microbiol. 2016; 7:142. PMC: 4749707. DOI: 10.3389/fmicb.2016.00142. View

14.

Rossolini G, DAndrea M, Mugnaioli C . The spread of CTX-M-type extended-spectrum beta-lactamases. Clin Microbiol Infect. 2007; 14 Suppl 1:33-41. DOI: 10.1111/j.1469-0691.2007.01867.x. View

15.

Mshana S, Falgenhauer L, Mirambo M, Mushi M, Moremi N, Julius R . Predictors of blaCTX-M-15 in varieties of Escherichia coli genotypes from humans in community settings in Mwanza, Tanzania. BMC Infect Dis. 2016; 16:187. PMC: 4850702. DOI: 10.1186/s12879-016-1527-x. View

16.

Bush K, Jacoby G . Updated functional classification of beta-lactamases. Antimicrob Agents Chemother. 2009; 54(3):969-76. PMC: 2825993. DOI: 10.1128/AAC.01009-09. View

17.

Marando R, Seni J, Mirambo M, Falgenhauer L, Moremi N, Mushi M . Predictors of the extended-spectrum-beta lactamases producing Enterobacteriaceae neonatal sepsis at a tertiary hospital, Tanzania. Int J Med Microbiol. 2018; 308(7):803-811. PMC: 6171784. DOI: 10.1016/j.ijmm.2018.06.012. View

18.

Mathur P . Hand hygiene: back to the basics of infection control. Indian J Med Res. 2011; 134(5):611-20. PMC: 3249958. DOI: 10.4103/0971-5916.90985. View

19.

Xia S, Fan X, Huang Z, Xia L, Xiao M, Chen R . Dominance of CTX-M-type extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from patients with community-onset and hospital-onset infection in China. PLoS One. 2014; 9(7):e100707. PMC: 4077569. DOI: 10.1371/journal.pone.0100707. View

20.

Asir J, Nair S, Devi S, Prashanth K, Saranathan R, Kanungo R . Simultaneous gut colonisation and infection by ESBL-producing Escherichia coli in hospitalised patients. Australas Med J. 2015; 8(6):200-7. PMC: 4496720. DOI: 10.4066/AMJ.2015.2358. View