Two VQ Proteins Are Substrates of the OsMPKK6-OsMPK4 Cascade in Rice Defense Against Bacterial Blight

Overview

Journal Rice (N Y)

Date 2021 Apr 29

PMID 33913048

Citations 11

Authors

Na Li

Zeyu Yang

Juan Li

Wenya Xie

Xiaofeng Qin

Yuanrong Kang

Qinglu Zhang

Xianghua Li

Jinghua Xiao

Haigang Ma

Shiping Wang

Affiliations

Soon will be listed here.

Abstract

Background: The plant-specific valine-glutamine (VQ) protein family with the conserved motif FxxxVQxLTG reportedly functions with the mitogen-activated protein kinase (MAPK) in plant immunity. However, the roles of VQ proteins in MAPK-mediated resistance to disease in rice remain largely unknown.

Results: In this study, two rice VQ proteins OsVQ14 and OsVQ32 were newly identified to function as the signaling components of a MAPK cascade, OsMPKK6-OsMPK4, to regulate rice resistance to Xanthomonas oryzae pv. oryzae (Xoo). Both OsVQ14 and OsVQ32 positively regulated rice resistance to Xoo. In vitro and in vivo studies revealed that OsVQ14 and OsVQ32 physically interacted with and were phosphorylated by OsMPK4. OsMPK4 was highly phosphorylated in transgenic plants overexpressing OsMPKK6, which showed enhanced resistance to Xoo. Meanwhile, phosphorylated OsVQ14 and OsVQ32 were also markedly accumulated in OsMPKK6-overexpressing transgenic plants.

Conclusions: We discovered that OsVQ14 and OsVQ32 functioned as substrates of the OsMPKK6-OsMPK4 cascade to enhance rice resistance to Xoo, thereby defining a more complete signal transduction pathway for induced defenses.

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