Comprehensive Description of the Current Breast Cancer Microenvironment Advancements Via Single-cell Analysis

Overview

Journal J Exp Clin Cancer Res

Publisher Biomed Central

Specialty Oncology

Date 2021 Apr 28

PMID 33906694

Citations 20

Authors

Xueqi Yan

Yinghong Xie

Fan Yang

Yijia Hua

Tianyu Zeng

Chunxiao Sun

Mengzhu Yang

Xiang Huang

Hao Wu

Ziyi Fu

Wei Li

Shiping Jiao

Yongmei Yin

Affiliations

Soon will be listed here.

Abstract

Breast cancer is a heterogeneous disease with a complex microenvironment consisting of tumor cells, immune cells, fibroblasts and vascular cells. These cancer-associated cells shape the tumor microenvironment (TME) and influence the progression of breast cancer and the therapeutic responses in patients. The exact composition of the intra-tumoral cells is mixed as the highly heterogeneous and dynamic nature of the TME. Recent advances in single-cell technologies such as single-cell DNA sequencing (scDNA-seq), single-cell RNA sequencing (scRNA-seq) and mass cytometry have provided new insights into the phenotypic and functional diversity of tumor-infiltrating cells in breast cancer. In this review, we have outlined the recent progress in single-cell characterization of breast tumor ecosystems, and summarized the phenotypic diversity of intra-tumoral cells and their potential prognostic relevance.

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References

Sinha P, Clements V, Ostrand-Rosenberg S . Interleukin-13-regulated M2 macrophages in combination with myeloid suppressor cells block immune surveillance against metastasis. Cancer Res. 2005; 65(24):11743-51. DOI: 10.1158/0008-5472.CAN-05-0045. View

Franklin R, Liao W, Sarkar A, Kim M, Bivona M, Liu K . The cellular and molecular origin of tumor-associated macrophages. Science. 2014; 344(6186):921-5. PMC: 4204732. DOI: 10.1126/science.1252510. View

Spranger S, Dai D, Horton B, Gajewski T . Tumor-Residing Batf3 Dendritic Cells Are Required for Effector T Cell Trafficking and Adoptive T Cell Therapy. Cancer Cell. 2017; 31(5):711-723.e4. PMC: 5650691. DOI: 10.1016/j.ccell.2017.04.003. View

Ijssennagger N, Janssen A, Milona A, Ramos Pittol J, Hollman D, Mokry M . Gene expression profiling in human precision cut liver slices in response to the FXR agonist obeticholic acid. J Hepatol. 2016; 64(5):1158-1166. DOI: 10.1016/j.jhep.2016.01.016. View

Chaffer C, Weinberg R . Cancer cell of origin: spotlight on luminal progenitors. Cell Stem Cell. 2010; 7(3):271-2. PMC: 2941878. DOI: 10.1016/j.stem.2010.08.008. View

Cohen N, Shani O, Raz Y, Sharon Y, Hoffman D, Abramovitz L . Fibroblasts drive an immunosuppressive and growth-promoting microenvironment in breast cancer via secretion of Chitinase 3-like 1. Oncogene. 2017; 36(31):4457-4468. PMC: 5507301. DOI: 10.1038/onc.2017.65. View

Wang Y, Mashock M, Tong Z, Mu X, Chen H, Zhou X . Changing Technologies of RNA Sequencing and Their Applications in Clinical Oncology. Front Oncol. 2020; 10:447. PMC: 7160325. DOI: 10.3389/fonc.2020.00447. View

Engblom C, Pfirschke C, Pittet M . The role of myeloid cells in cancer therapies. Nat Rev Cancer. 2016; 16(7):447-62. DOI: 10.1038/nrc.2016.54. View

Snell L, McGaha T, Brooks D . Type I Interferon in Chronic Virus Infection and Cancer. Trends Immunol. 2017; 38(8):542-557. PMC: 8059441. DOI: 10.1016/j.it.2017.05.005. View

10.

Macosko E, Basu A, Satija R, Nemesh J, Shekhar K, Goldman M . Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets. Cell. 2015; 161(5):1202-1214. PMC: 4481139. DOI: 10.1016/j.cell.2015.05.002. View

11.

Hamzah J, Jugold M, Kiessling F, Rigby P, Manzur M, Marti H . Vascular normalization in Rgs5-deficient tumours promotes immune destruction. Nature. 2008; 453(7193):410-4. DOI: 10.1038/nature06868. View

12.

Bartoschek M, Oskolkov N, Bocci M, Lovrot J, Larsson C, Sommarin M . Spatially and functionally distinct subclasses of breast cancer-associated fibroblasts revealed by single cell RNA sequencing. Nat Commun. 2018; 9(1):5150. PMC: 6279758. DOI: 10.1038/s41467-018-07582-3. View

13.

Sautes-Fridman C, Petitprez F, Calderaro J, Fridman W . Tertiary lymphoid structures in the era of cancer immunotherapy. Nat Rev Cancer. 2019; 19(6):307-325. DOI: 10.1038/s41568-019-0144-6. View

14.

McAllister S, Weinberg R . Tumor-host interactions: a far-reaching relationship. J Clin Oncol. 2010; 28(26):4022-8. DOI: 10.1200/JCO.2010.28.4257. View

15.

Jiang Y, Li Y, Zhu B . T-cell exhaustion in the tumor microenvironment. Cell Death Dis. 2015; 6:e1792. PMC: 4669840. DOI: 10.1038/cddis.2015.162. View

16.

Gallego-Ortega D, Ledger A, Roden D, Law A, Magenau A, Kikhtyak Z . ELF5 Drives Lung Metastasis in Luminal Breast Cancer through Recruitment of Gr1+ CD11b+ Myeloid-Derived Suppressor Cells. PLoS Biol. 2015; 13(12):e1002330. PMC: 4696735. DOI: 10.1371/journal.pbio.1002330. View

17.

Coates A, Winer E, Goldhirsch A, Gelber R, Gnant M, Piccart-Gebhart M . Tailoring therapies--improving the management of early breast cancer: St Gallen International Expert Consensus on the Primary Therapy of Early Breast Cancer 2015. Ann Oncol. 2015; 26(8):1533-46. PMC: 4511219. DOI: 10.1093/annonc/mdv221. View

18.

Vivier E, Raulet D, Moretta A, Caligiuri M, Zitvogel L, Lanier L . Innate or adaptive immunity? The example of natural killer cells. Science. 2011; 331(6013):44-9. PMC: 3089969. DOI: 10.1126/science.1198687. View

19.

De Haas N, de Koning C, Spilgies L, de Vries I, Hato S . Improving cancer immunotherapy by targeting the STATe of MDSCs. Oncoimmunology. 2016; 5(7):e1196312. PMC: 5006927. DOI: 10.1080/2162402X.2016.1196312. View

20.

Cremasco V, Astarita J, Grauel A, Keerthivasan S, MacIsaac K, Woodruff M . FAP Delineates Heterogeneous and Functionally Divergent Stromal Cells in Immune-Excluded Breast Tumors. Cancer Immunol Res. 2018; 6(12):1472-1485. PMC: 6597261. DOI: 10.1158/2326-6066.CIR-18-0098. View