Proteomic and Transcriptomic Analyses Reveal Pathological Changes in the Entorhinal Cortex Region That Correlate Well with Dysregulation of Ion Transport in Patients with Alzheimer's Disease

Overview

Journal Mol Neurobiol

Specialties Molecular Biology
Neurology

Date 2021 Apr 27

PMID 33904022

Citations 10

Authors

Yangjie Jia

Xia Wang

Yanyu Chen

Wenying Qiu

Wei Ge

Chao Ma

Affiliations

Soon will be listed here.

Abstract

Alzheimer's disease (AD) is the most common neurodegenerative disorder. The earliest neuropathology of AD appears in entorhinal cortex (EC) regions. Therapeutic strategies and preventive measures to protect against entorhinal degeneration would be of substantial value in the early stages of AD. In this study, transcriptome based on the Illumina RNA-seq and proteome based on TMT-labelling were performed for RNA and protein profiling on AD EC samples and non-AD control EC samples. Immunohistochemistry was used to validate proteins expressions. After integrated analysis, 57 genes were detected both in transcriptome and proteome data, including 51 in similar altering trends (7 upregulated, 44 downregulated) and 6 in inverse trends when compared AD vs. control. The top 6 genes (GABRG2, CACNG3, CACNB4, GABRB2, GRIK2, and SLC17A6) within the 51 genes were selected and related to "ion transport". Correlation analysis demonstrated negative relationship of protein expression level with the neuropathologic changes. In conclusion, the integrate transcriptome and proteome analysis provided evidence for dysregulation of ion transport across brain regions in AD, which might be a critical signaling pathway that initiates pathology. This study might provide new insight into the earliest changes occurring in the EC of AD and novel targets for AD prevention and treatment.

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