Cellular-resolution Gene Expression Profiling in the Neonatal Marmoset Brain Reveals Dynamic Species- and Region-specific Differences

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2021 Apr 27

PMID 33903237

Citations 15

Authors

Yoshiaki Kita

Hirozumi Nishibe

Yan Wang

Tsutomu Hashikawa

Satomi S Kikuchi

Mami U

Aya C Yoshida

Chihiro Yoshida

Takashi Kawase

Shin Ishii

Henrik Skibbe

Tomomi Shimogori

Affiliations

Soon will be listed here.

Abstract

Precise spatiotemporal control of gene expression in the developing brain is critical for neural circuit formation, and comprehensive expression mapping in the developing primate brain is crucial to understand brain function in health and disease. Here, we developed an unbiased, automated, large-scale, cellular-resolution in situ hybridization (ISH)-based gene expression profiling system (GePS) and companion analysis to reveal gene expression patterns in the neonatal New World marmoset cortex, thalamus, and striatum that are distinct from those in mice. Gene-ontology analysis of marmoset-specific genes revealed associations with catalytic activity in the visual cortex and neuropsychiatric disorders in the thalamus. Cortically expressed genes with clear area boundaries were used in a three-dimensional cortical surface mapping algorithm to delineate higher-order cortical areas not evident in two-dimensional ISH data. GePS provides a powerful platform to elucidate the molecular mechanisms underlying primate neurobiology and developmental psychiatric and neurological disorders.

Citing Articles

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