Evaluation of a Direct Reverse Transcription Loop-Mediated Isothermal Amplification Method Without RNA Extraction (Direct RT-LAMP) for the Detection of Lymph Node Metastasis in Early Breast Cancer

Overview

Journal Cancer Res Treat

Specialty Oncology

Date 2021 Apr 26

PMID 33902166

Citations 1

Authors

In Hee Lee

Jin Hyang Jung

Soo Jung Lee

Jeeyeon Lee

Ho Yong Park

Ji-Young Park

Jee Young Park

Jae-Hwan Jung

Hyunchul Lee

Hyo-Sung Jeon

Yee Soo Chae

Affiliations

Soon will be listed here.

Abstract

Purpose: Assessing lymph node metastasis, tumor-derived DNA, or tumor-derived RNA has previously been studied in place of immunohistochemical assay. Because a direct reverse transcription loop-mediated isothermal amplification method (direct RT-LAMP) has been previously developed in order to rapidly identify viruses in place of RNA extraction, our team hypothesized that a direct RT-LAMP assay can be employed as a substitute in order to detect tumor involvement of lymph nodes within breast cancer patients.

Materials And Methods: A total amount of 92 lymph nodes removed across 40 patients possessing breast cancer were collected at Kyungpook National University Chilgok Hospital between the months of November 2015 and February 2016. All samples were then evaluated and contrasted via both a direct RT-LAMP assay and routine histopathologic examination.

Results: The sensitivity and specificity of the direct RT-LAMP assay were 85.7% and 100%, respectively. The positive predictive value and negative predictive value were 100% and 94.4%, respectively.

Conclusion: Direct RT-LAMP assay is capable of facilitating the detection of sentinel lymph node metastasis within breast cancer patients intraoperatively possessing an excellent sensitivity via a cost-effective and time-saving manner.

Citing Articles

Versatility of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) from diagnosis of early pathological infection to mutation detection in organisms.

Sen S, Bhowmik P, Tiwari S, Peleg Y, Bandyopadhyay B Mol Biol Rep. 2024; 51(1):211.

PMID: 38270670 DOI: 10.1007/s11033-023-09110-z.

References

Szychta P, Westfal B, Maciejczyk R, Smolarz B, Romanowicz H, Krawczyk T . Intraoperative diagnosis of sentinel lymph node metastases in breast cancer treatment with one-step nucleic acid amplification assay (OSNA). Arch Med Sci. 2016; 12(6):1239-1246. PMC: 5108387. DOI: 10.5114/aoms.2016.62902. View

Mamounas E, Brown A, Anderson S, Smith R, Julian T, Miller B . Sentinel node biopsy after neoadjuvant chemotherapy in breast cancer: results from National Surgical Adjuvant Breast and Bowel Project Protocol B-27. J Clin Oncol. 2005; 23(12):2694-702. DOI: 10.1200/JCO.2005.05.188. View

Shi F, Liang Z, Zhang Q, Wang C, Liu X . The performance of one-step nucleic acid amplification assay for intraoperative detection of sentinel lymph node macrometastasis in breast cancer: An updated meta-analysis. Breast. 2018; 39:39-45. DOI: 10.1016/j.breast.2018.03.005. View

Giuliano A, Dale P, Turner R, Morton D, Evans S, Krasne D . Improved axillary staging of breast cancer with sentinel lymphadenectomy. Ann Surg. 1995; 222(3):394-9; discussion 399-401. PMC: 1234825. DOI: 10.1097/00000658-199509000-00016. View

Nie K, Qi S, Zhang Y, Luo L, Xie Y, Yang M . Evaluation of a direct reverse transcription loop-mediated isothermal amplification method without RNA extraction for the detection of human enterovirus 71 subgenotype C4 in nasopharyngeal swab specimens. PLoS One. 2012; 7(12):e52486. PMC: 3525532. DOI: 10.1371/journal.pone.0052486. View

Tanis P, Boom R, Koops H, Faneyte I, Peterse J, Nieweg O . Frozen section investigation of the sentinel node in malignant melanoma and breast cancer. Ann Surg Oncol. 2001; 8(3):222-6. DOI: 10.1007/s10434-001-0222-2. View

Smolarz B, Krawczyk T, Westfal B, Maciejczyk R, Zadrozny M, Samulak D . Comparison of one-step nucleic acid amplification (OSNA) method and routine histological investigation for intraoperative detection of lymph node metastasis in Polish women with breast cancer. Pol J Pathol. 2013; 64(2):104-8. DOI: 10.5114/pjp.2013.36014. View

Liu L, Lang J, Lu Y, Roe D, Hwang S, Ewing C . Intraoperative frozen section analysis of sentinel lymph nodes in breast cancer patients: a meta-analysis and single-institution experience. Cancer. 2010; 117(2):250-8. DOI: 10.1002/cncr.25606. View

Cserni G . Intraoperative analysis of sentinel lymph nodes in breast cancer by one-step nucleic acid amplification. J Clin Pathol. 2011; 65(3):193-9. DOI: 10.1136/jclinpath-2011-200301. View

10.

Nie K, Zhang Y, Luo L, Yang M, Hu X, Wang M . Visual detection of human enterovirus 71 subgenotype C4 and Coxsackievirus A16 by reverse transcription loop-mediated isothermal amplification with the hydroxynaphthol blue dye. J Virol Methods. 2011; 175(2):283-6. DOI: 10.1016/j.jviromet.2011.05.020. View

11.

Hsieh K, Mage P, Csordas A, Eisenstein M, Soh H . Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP). Chem Commun (Camb). 2014; 50(28):3747-9. DOI: 10.1039/c4cc00540f. View

12.

Tsujimoto M, Nakabayashi K, Yoshidome K, Kaneko T, Iwase T, Akiyama F . One-step nucleic acid amplification for intraoperative detection of lymph node metastasis in breast cancer patients. Clin Cancer Res. 2007; 13(16):4807-16. DOI: 10.1158/1078-0432.CCR-06-2512. View

13.

Fleissig A, Fallowfield L, Langridge C, Johnson L, Newcombe R, Dixon J . Post-operative arm morbidity and quality of life. Results of the ALMANAC randomised trial comparing sentinel node biopsy with standard axillary treatment in the management of patients with early breast cancer. Breast Cancer Res Treat. 2005; 95(3):279-93. DOI: 10.1007/s10549-005-9025-7. View

14.

Krag D, Anderson S, Julian T, Brown A, Harlow S, Costantino J . Sentinel-lymph-node resection compared with conventional axillary-lymph-node dissection in clinically node-negative patients with breast cancer: overall survival findings from the NSABP B-32 randomised phase 3 trial. Lancet Oncol. 2010; 11(10):927-33. PMC: 3041644. DOI: 10.1016/S1470-2045(10)70207-2. View

15.

Tew K, Irwig L, Matthews A, Crowe P, Macaskill P . Meta-analysis of sentinel node imprint cytology in breast cancer. Br J Surg. 2005; 92(9):1068-80. DOI: 10.1002/bjs.5139. View

16.

Mohon A, Oberding L, Hundt J, van Marle G, Pabbaraju K, Berenger B . Optimization and clinical validation of dual-target RT-LAMP for SARS-CoV-2. J Virol Methods. 2020; 286:113972. PMC: 7490281. DOI: 10.1016/j.jviromet.2020.113972. View