Aurora Kinase A is Essential for Meiosis in Mouse Oocytes

Overview

Journal PLoS Genet

Specialty Genetics

Date 2021 Apr 26

PMID 33901174

Citations 32

Authors

Cecilia S Blengini

Patricia Ibrahimian

Michaela Vaskovicova

David Drutovic

Petr Solc

Karen Schindler

Affiliations

Soon will be listed here.

Abstract

The Aurora protein kinases are well-established regulators of spindle building and chromosome segregation in mitotic and meiotic cells. In mouse oocytes, there is significant Aurora kinase A (AURKA) compensatory abilities when the other Aurora kinase homologs are deleted. Whether the other homologs, AURKB or AURKC can compensate for loss of AURKA is not known. Using a conditional mouse oocyte knockout model, we demonstrate that this compensation is not reciprocal because female oocyte-specific knockout mice are sterile, and their oocytes fail to complete meiosis I. In determining AURKA-specific functions, we demonstrate that its first meiotic requirement is to activate Polo-like kinase 1 at acentriolar microtubule organizing centers (aMTOCs; meiotic spindle poles). This activation induces fragmentation of the aMTOCs, a step essential for building a bipolar spindle. We also show that AURKA is required for regulating localization of TACC3, another protein required for spindle building. We conclude that AURKA has multiple functions essential to completing MI that are distinct from AURKB and AURKC.

Citing Articles

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