MiR-375 Promotes Pancreatic Differentiation by Affecting Different Target Genes at Different Stages

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2021 Apr 26

PMID 33897780

Citations 6

Authors

Zhenyu Lu

Jing Wang

Xu Wang

Zhiying Li

Dan Niu

Min Wang

Jinzhu Xiang

Yongli Yue

Yajuan Xia

Xueling Li

Affiliations

Soon will be listed here.

Abstract

Human embryonic stem cells (hESCs) possess the ability to differentiate into insulin-producing cells (IPCs), which can be used to treat type I diabetes. miR-375 is an essential transcriptional regulator in the development and maturation of the pancreas. In this study, we optimized a protocol to differentiate hESCs into IPCs and successfully obtained IPCs. Then, we performed overexpression and inhibition experiments of miR-375 on cells at different stages of differentiation and performed RNA-seq. The results showed that the expression of miR-375 fluctuated during hESC differentiation and was affected by miR-375 mimics and inhibitors. miR-375 influences global gene expression and the target genes of miR-375. The overexpression of miR-375 can cause changes in multiple signaling pathways during pancreatic development. miR-375 is a major participant in the differentiation of pancreatic -cells through different target genes at different stages. This study provides new ideas for further investigation of how microRNAs affect cell fate and gene transcription.

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