Advances and Perspectives for Genome Editing Tools of

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Apr 26

PMID 33897668

Citations 15

Authors

Qingzhuo Wang

Jiao Zhang

Naief H Al Makishah

Xiaoman Sun

Zhiqiang Wen

Yu Jiang

Sheng Yang

Affiliations

Soon will be listed here.

Abstract

has been considered a promising synthetic biological platform for biomanufacturing and bioremediation. However, there are still some challenges in genetic manipulation of . Recently, more and more genetic parts or elements (replicons, promoters, reporter genes, and selectable markers) have been mined, characterized, and applied. In addition, continuous improvement of classic molecular genetic manipulation techniques, such as allelic exchange via single/double-crossover, nuclease-mediated site-specific recombination, RecT-mediated single-chain recombination, actinophages integrase-mediated integration, and transposition mutation, has accelerated the molecular study of More importantly, emerging gene editing tools based on the CRISPR/Cas system is revolutionarily rewriting the pattern of genetic manipulation technology development for , which made gene reprogramming, such as insertion, deletion, replacement, and point mutation, much more efficient and simpler. This review summarized the recent progress in molecular genetic manipulation technology development of and discussed the bottlenecks and perspectives for future research of as a distinctive microbial chassis.

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