Targets and Mechanisms of Geminivirus Silencing Suppressor Protein AC2

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Apr 26

PMID 33897657

Citations 7

Authors

Karuppannan Veluthambi

Sukumaran Sunitha

Affiliations

Soon will be listed here.

Abstract

Geminiviruses are plant DNA viruses that infect a wide range of plant species and cause significant losses to economically important food and fiber crops. The single-stranded geminiviral genome encodes a small number of proteins which act in an orchestrated manner to infect the host. The fewer proteins encoded by the virus are multifunctional, a mechanism uniquely evolved by the viruses to balance the genome-constraint. The host-mediated resistance against incoming virus includes post-transcriptional gene silencing, transcriptional gene silencing, and expression of defense responsive genes and other cellular regulatory genes. The pathogenicity property of a geminiviral protein is linked to its ability to suppress the host-mediated defense mechanism. This review discusses what is currently known about the targets and mechanism of the viral suppressor AC2/AL2/transcriptional activator protein (TrAP) and explore the biotechnological applications of AC2.

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References

Raja P, Jackel J, Li S, Heard I, Bisaro D . Arabidopsis double-stranded RNA binding protein DRB3 participates in methylation-mediated defense against geminiviruses. J Virol. 2013; 88(5):2611-22. PMC: 3958096. DOI: 10.1128/JVI.02305-13. View

Aregger M, Borah B, Seguin J, Rajeswaran R, Gubaeva E, Zvereva A . Primary and secondary siRNAs in geminivirus-induced gene silencing. PLoS Pathog. 2012; 8(9):e1002941. PMC: 3460622. DOI: 10.1371/journal.ppat.1002941. View

Shivaprasad P, Akbergenov R, Trinks D, Rajeswaran R, Veluthambi K, Hohn T . Promoters, transcripts, and regulatory proteins of Mungbean yellow mosaic geminivirus. J Virol. 2005; 79(13):8149-63. PMC: 1143740. DOI: 10.1128/JVI.79.13.8149-8163.2005. View

Robertson D . VIGS vectors for gene silencing: many targets, many tools. Annu Rev Plant Biol. 2004; 55:495-519. DOI: 10.1146/annurev.arplant.55.031903.141803. View

Li P, Liu C, Deng W, Yao D, Pan L, Li Y . Plant begomoviruses subvert ubiquitination to suppress plant defenses against insect vectors. PLoS Pathog. 2019; 15(2):e1007607. PMC: 6400417. DOI: 10.1371/journal.ppat.1007607. View

Daxinger L, Kanno T, Bucher E, van der Winden J, Naumann U, Matzke A . A stepwise pathway for biogenesis of 24-nt secondary siRNAs and spreading of DNA methylation. EMBO J. 2008; 28(1):48-57. PMC: 2633084. DOI: 10.1038/emboj.2008.260. View

Chung H, Lacatus G, Sunter G . Geminivirus AL2 protein induces expression of, and interacts with, a calmodulin-like gene, an endogenous regulator of gene silencing. Virology. 2014; 460-461:108-18. DOI: 10.1016/j.virol.2014.04.034. View

Sunter G, Stenger D, Bisaro D . Heterologous complementation by geminivirus AL2 and AL3 genes. Virology. 1994; 203(2):203-10. DOI: 10.1006/viro.1994.1477. View

Akbergenov R, Si-Ammour A, Blevins T, Amin I, Kutter C, Vanderschuren H . Molecular characterization of geminivirus-derived small RNAs in different plant species. Nucleic Acids Res. 2006; 34(2):462-71. PMC: 1342034. DOI: 10.1093/nar/gkj447. View

10.

Krenz B, Deuschle K, Deigner T, Unseld S, Kepp G, Wege C . Early function of the Abutilon mosaic virus AC2 gene as a replication brake. J Virol. 2015; 89(7):3683-99. PMC: 4403429. DOI: 10.1128/JVI.03491-14. View

11.

Dong X, van Wezel R, Stanley J, Hong Y . Functional characterization of the nuclear localization signal for a suppressor of posttranscriptional gene silencing. J Virol. 2003; 77(12):7026-33. PMC: 156164. DOI: 10.1128/jvi.77.12.7026-7033.2003. View

12.

Garcia-Ruiz H, Takeda A, Chapman E, Sullivan C, Fahlgren N, Brempelis K . Arabidopsis RNA-dependent RNA polymerases and dicer-like proteins in antiviral defense and small interfering RNA biogenesis during Turnip Mosaic Virus infection. Plant Cell. 2010; 22(2):481-96. PMC: 2845422. DOI: 10.1105/tpc.109.073056. View

13.

Yang X, Baliji S, Buchmann R, Wang H, Lindbo J, Sunter G . Functional modulation of the geminivirus AL2 transcription factor and silencing suppressor by self-interaction. J Virol. 2007; 81(21):11972-81. PMC: 2168806. DOI: 10.1128/JVI.00617-07. View

14.

Zhang H, Lang Z, Zhu J . Dynamics and function of DNA methylation in plants. Nat Rev Mol Cell Biol. 2018; 19(8):489-506. DOI: 10.1038/s41580-018-0016-z. View

15.

Padidam M, Beachy R, Fauquet C . The role of AV2 ("precoat") and coat protein in viral replication and movement in tomato leaf curl geminivirus. Virology. 1996; 224(2):390-404. DOI: 10.1006/viro.1996.0546. View

16.

White D . PEAPOD regulates lamina size and curvature in Arabidopsis. Proc Natl Acad Sci U S A. 2006; 103(35):13238-43. PMC: 1550771. DOI: 10.1073/pnas.0604349103. View

17.

Holler K, Kiraly L, Kunstler A, Muller M, Gullner G, Fattinger M . Enhanced glutathione metabolism is correlated with sulfur-induced resistance in Tobacco mosaic virus-infected genetically susceptible Nicotiana tabacum plants. Mol Plant Microbe Interact. 2010; 23(11):1448-59. DOI: 10.1094/MPMI-05-10-0117. View

18.

van Wezel R, Liu H, Tien P, Stanley J, Hong Y . Gene C2 of the monopartite geminivirus tomato yellow leaf curl virus-China encodes a pathogenicity determinant that is localized in the nucleus. Mol Plant Microbe Interact. 2001; 14(9):1125-8. DOI: 10.1094/MPMI.2001.14.9.1125. View

19.

Sunter G, Bisaro D . Transactivation of geminivirus AR1 and BR1 gene expression by the viral AL2 gene product occurs at the level of transcription. Plant Cell. 1992; 4(10):1321-31. PMC: 160218. DOI: 10.1105/tpc.4.10.1321. View

20.

Raja P, Wolf J, Bisaro D . RNA silencing directed against geminiviruses: post-transcriptional and epigenetic components. Biochim Biophys Acta. 2010; 1799(3-4):337-51. DOI: 10.1016/j.bbagrm.2010.01.004. View